지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Considering the greater role of α-amino acids in our daily lives, the enantiomer resolution of seven α-amino acids derivatized with fluorogenic reagent (4-fluoro-7-nitro-2,1,3-benzoxadiazole, NBD-F) by chiral HPLC on amylose or cellulose trisphenylcarbamate derived chiral stationary phases (CSPs) under simultaneous ultraviolet (UV) and fluorescence (FL) detection was performed. The degree of enantioseparation and resolution was affected by nature and selector backbones of the CSPs as well as the kind of amino acids. Baseline enantiomer separation and resolutions were observed for the enantiomers of all analytes as NBD derivatives especially on coated type amylose tris(3,5-dimethylphenylcarbamate) derived CSPs (Chiralpak AD-H and Lux Amylose-1). The other CSPs also showed good enantioselectivity except for the CSPs (Chiralpak IB, Chiralcel OD-H and Lux Cellulose-1) having cellulose tris(3,5-dimethylphenylcarbamate) as chiral selectors. The developed analytical chiral method was applied to determine the enantiomeric purity of seven commercially available L-α-amino acids and the impurities as D-forms were found to be in the range 0.08-0.87 %, respectively. The intra- and interday accuracy and precision assays showed high accuracy and precision of the developed analytical method. This chiral HPLC method for the enantiomer resolution of amino acids using fluorescent derivatization could be useful for the determination of enantiomeric purity of pharmaceuticals and biological study for amino acid type compounds among chiral drugs.
목차
Abstract
1. Introduction
2. Experimental
3. Results and Discussion
4. Conclusions
References
참고문헌 (29)
참고문헌 신청
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G. Subramanian, ‘Chiral Separation Techniques: A Practical Approach’, 3rd Ed., Wiley-VCH, Germany, 2006.
A. Bongioanni, M. S. Bueno, B. A. Mezzano, M. R. Longhi, and C. Garnero, Int. J. Pharm., 613, 121375-121387 (2022). https://doi.org/10.1016/j.ijpharm.2021.121375
K. Kupnik, Z. Knez, M. Primožič, and M. Leitgeb, Sep. Purif. Rev., 52(1), 58-74 (2023). https://doi.org/10.1080/15422119.2022.2038625
I. Molnár-Perl, ‘Quantitation of Amino Acids and Amines by Chromatography: Methods and Protocols’, Elsevier Science, Amsterdam, 2005.
G. Subramanian, ‘Chiral Separation Techniques: A Practical Approach’, 3rd Ed., Wiley-VCH, Germany, 2006.
A. Bongioanni, M. S. Bueno, B. A. Mezzano, M. R. Longhi, and C. Garnero, Int. J. Pharm., 613, 121375-121387 (2022). https://doi.org/10.1016/j.ijpharm.2021.121375
K. Kupnik, Z. Knez, M. Primožič, and M. Leitgeb, Sep. Purif. Rev., 52(1), 58-74 (2023). https://doi.org/10.1080/15422119.2022.2038625
I. Molnár-Perl, ‘Quantitation of Amino Acids and Amines by Chromatography: Methods and Protocols’, Elsevier Science, Amsterdam, 2005.
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UCI(KEPA) : I410-ECN-0102-2023-433-001946342