Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing

Defu Wang; Liyan Cui; Yanni Pei; Zhennan Ma; Shaofei Shen; Dandan Long; Lingyu Li; Yanbing Niu

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자료유형: 학술저널

저자정보: Defu Wang (Shanxi Agricultural University) Liyan Cui (Shanxi Agricultural University) Yanni Pei (Shanxi Agricultural University) Zhennan Ma (Shanxi Agricultural University) Shaofei Shen (Shanxi Agricultural University) Dandan Long (Shanxi Agricultural University) Lingyu Li (Shanxi Agricultural University) Yanbing Niu (Shanxi Agricultural University)

저널정보: 한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal 제36권 제5호

발행연도: 2020.10

수록면: 468 - 475 (8page)

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Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCVSX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids. Phylogenetic analysis showed that MVCVSX was clustered with euphorbia ringspot virus and yam mosaic virus. Further analyses of the vsiR profiles revealed that the most abundant MVCV-vsiRs were between 21 and 22 nucleotides in length and a strong bias was found for “A” and “U” at the 5′-terminal residue. The results of polarity assessment indicated that the amount of sense strand was almost equal to that of the antisense strand in MVCV-vsiRs, and the main hot-spot region in MVCV-SX genome was found at cylindrical inclusion. In conclusion, our findings could provide new insights into the RNA silencing-mediated host defence mechanism in A. rosea infected with MVCV-SX, and offer a basis for the prevention and treatment of this virus disease.

#Alcea rosea (L.) #malva vein clearing virus #small RNA sequencing #virus-derived small interfering RNA

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