In Vitro Wheat Immature Spike Culture Screening Identified Fusarium Head Blight Resistance in Wheat Spike Cultured Derived Variants and in the Progeny of Their Crosses with an Elite Cultivar

Chen Huang; Manu P. Gangola; H. Randy Kutcher; Pierre Hucl; Seedhabadee Ganeshan; Ravindra N. Chibbar

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저자정보: Chen Huang (University of Saskatchewan) Manu P. Gangola (University of Saskatchewan) H. Randy Kutcher (University of Saskatchewan) Pierre Hucl (University of Saskatchewan) Seedhabadee Ganeshan (University of Saskatchewan) Ravindra N. Chibbar (University of Saskatchewan)

저널정보: 한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal 제36권 제6호

발행연도: 2020.12

수록면: 558 - 569 (12page)

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Fusarium head blight (FHB) is a devastating fungal disease of wheat (Triticum aestivum L.). The lack of genetic resources with stable FHB resistance combined with a reliable and rapid screening method to evaluate FHB resistance is a major limitation to the development of FHB resistant wheat germplasm. The present study utilized an immature wheat spike culture method to screen wheat spike culture derived variants (SCDV) for FHB resistance. Mycotoxin concentrations determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) correlated significantly (P < 0.01) with FHB severity and disease progression during in vitro spike culture. Selected SCDV lines assessed for FHB resistance in a Fusarium field disease nursery in Carman, Manitoba, Canada in 2016 showed significant (P < 0.01) correlation of disease severity to the in vitro spike culture screening method. Selected resistant SCDV lines were also crossed with an elite cv. CDC Hughes and the progeny of F2 and BC1F2 were screened by high resolution melt curve (HRM) analyses for the wheat UDP-glucosyl transferase gene (TaUGT-3B) single nucleotide polymorphism to identify resistant (T-allele) and susceptible (G-allele) markers. The progeny from the crosses were also screened for FHB severity using the immature spike culture method and identified resistant progeny grouped according to the HRM genotyping data. The results demonstrate a reliable approach using the immature spike culture to screen for FHB resistance in progeny of crosses in early stage of breeding programs.

#3-acetyldeoxynivalenol #deoxynivalenol #deoxynivalenol-3-glucoside #Fusarium head blight #wheat

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