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Seeds of wheat (Triticum aestivum L. cv. Olgeurumil) were infected with Penicillium sp. at mean infection rate of 83%. Penicillium sp. was detected in endosperm with bran but not in embryo. Gaseous chlorine dioxide (ClO<sub>2</sub>) effectively inhibited growth of Penicillium sp. at concentration of 5 to 20 mg/ml. As treatment duration was extended from 1 to 3 h, growth of Penicillium sp. was completely suppressed even at 10 mg/ml. There was no significant reduction in the incidence of Penicillium sp. at 30% relative humidity (RH). However, the incidence of Penicillium sp. was 27.7% at 50% RH, further those were 3.5% and 0.2% at 70% and 80% RH, respectively. Seed germination was not affected by ClO<sub>2</sub> treatment at all the RH conditions. Water-soaked seeds (30% seed moisture content) showed a drastic reduction in the incidence of Penicillium sp. when treated at more than 10 mg/ml of ClO<sub>2</sub>. The incidences of Penicillium sp. were 3.3, 1.8 and 1.2% at 10, 15 and 20 mg/ml, respectively. The incidence of Penicillium sp. in dry seeds with 9.7% seed moisture content did not reduce when treated with 5 and 10 mg/ml at 50% RH although it tended to decrease as ClO<sub>2</sub> concentration increased to 20 mg/ml. Seed germination was not affected by ClO<sub>2</sub> treatment at the tested concentrations. These results indicated that gaseous ClO<sub>2</sub> was effective disinfectant to wheat seeds infected with Penicillium sp. and that the effectiveness of ClO<sub>2</sub> strongly increased when moisture content around or inside of the seed was increased.

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