지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 379 - 387 (9page)
이용수
초록· 키워드
오류제보하기
Melon necrotic spot virus (MNSV) was recently identi- fied on watermelon (Citrullus vulgaris) in Korea, dis- playing as large necrotic spots and vein necrosis on the leaves and stems. The average occurrence of MNSV on watermelon was found to be 30-65% in Hapcheon and Andong City, respectively. Four isolates of the virus (MNSV-HW, MNSV-AW, MNSV-YW, and MNSV-SW) obtained from watermelon plants in different areas were non-pathogenic on ten general indicator plants, including Chenopodium quinoa, while they infected systemically six varieties of Cucurbitaceae. The virus particles purified by 10-40% sucrose density gradient centrifugation had a typical ultraviolet spectrum, with a minimum at 245 nm and a maximum at 260 nm. The morphology of the virus was spherical with a diameter of 28-30 nm. Virus particles were observed scattered throughout the cytoplasm of watermelon cells, but no crystals were detected. An ELISA was conducted using antiserum against MNSV-HW; the optimum concentrations of IgG and conjugated IgG for the assay were 1 μl/ml and a 1:8,000-1:10,000 dilutions, respectively. Antiserum against MNSV-HW could capture specifically both MNSV-MN from melon and MNSV-HW from watermelon by IC/RT-PCR, and they were effectively detected with the same specific primer to produce product of 1,172 bp. The dsRNA of MNSV-HW had the same profile (4.5, 1.8, and 1.6 kb) as that of MNSV-MN from melon. The nucleotide sequence of the coat protein of MNSV-HW gave a different phylogenetic tree, having 17.2% difference in nucleotide sequence compared with MNSV isolates from melon.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
국내 수박, 멜론 감염 Zucchini Yellow Mosaic Virus, Watermelon Mosaic Virus, Melon Necrotic Spot Virus의 현황 조사 및 유전적 연구
Research in Plant Disease(식물병연구)
2024 .12
-
Development of a Multiplex Polymerase Chain Reaction Assay for Detecting Five Previously Unreported Papaya Viruses for Quarantine Purposes in Korea
Research in Plant Disease(식물병연구)
2024 .09
-
Comprehensive Metatranscriptomic Analysis of Plant Viruses in Imported Frozen Cherries and Blueberries
The Plant Pathology Journal
2024 .08
-
Identification of Plant Viruses Infecting Pear Using RNA Sequencing
The Plant Pathology Journal
2021 .06
-
The Plant Cellular Systems for Plant Virus Movement
The Plant Pathology Journal
2017 .06
-
주거복지 인식에 따른 청년일자리로서의 주거복지사 탐색
한국주거학회 학술대회논문집
2019 .11
-
Detection of Plant Pathogenic Viruses in Commercial Gochujang (Fermented Red Pepper Paste) from Korea
The Plant Pathology Journal
2020 .10
-
An Inexpensive System for Rapid and Accurate On-site Detection of Garlic-Infected Viruses by Agarose Gel Electrophoresis Followed by Array Assay
The Plant Pathology Journal
2024 .02
-
Korea Bank for Pathogenic Viruses
한국미생물학회 학술대회논문집
2015 .04
-
Identification of Leonurus sibiricus as a Weed Reservoir for Three Pepper-Infecting Viruses
The Plant Pathology Journal
2016 .02
-
Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants
Research in Plant Disease(식물병연구)
2018 .09
-
Incidence of Viral Diseases and Occurrence of Three Unreported Viruses in Yams in Korea
Research in Plant Disease(식물병연구)
2017 .03
-
Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants
Research in Plant Disease(식물병연구)
2018 .12
-
국내 초석잠에 복합 감염된 3종 바이러스의 분리 및 동정
Research in Plant Disease(식물병연구)
2018 .03
-
Structure and Function of the Influenza A Virus Non-Structural Protein 1
Journal of Microbiology and Biotechnology
2019 .01
-
Computation of Refractive Indices of Corona Viruses through Reverse Calculation
Current Optics and Photonics
2020 .12
-
Pathogenesis of Oncoviruses: A Systemic Review
한국미생물·생명공학회지
2023 .06
-
Complete Sequence Analysis of a Korean Isolate of Chinese Yam Necrotic Mosaic Virus and Generation of the Virus Specific Primers for Molecular Detection
Research in Plant Disease(식물병연구)
2016 .09
-
Metatranscriptomic Analysis of Plant Viruses in Imported Pear and Kiwifruit Pollen
The Plant Pathology Journal
2022 .06
-
The Detection of Plant Viruses in Korean Ginseng (Panax ginseng) through RNA Sequencing
The Plant Pathology Journal
2020 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0102-2023-481-001833915