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논문 기본 정보

자료유형
학술저널
저자정보
Young-Joo Yi (Sunchon National University) Wijesooriya Mudhiyanselage Nadeema Dissanayake (Sunchon National University) Ram Prasad Aganja (Jeonbuk National University) Sang-Myeong Lee (Chungbuk National University)
저널정보
충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.50 No.1
발행연도
2023.3
수록면
131 - 139 (9page)

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Shilajit is a dark-brown exudate found in the rock formations of the Himalayas and contains various minerals, vitamins, and enzymes. The present study was performed to examine sperm motility, sperm adenosine triphosphate (ATP) content, generation of reactive oxygen species (ROS), and calcium ion (Ca<SUP>2+</SUP>) levels after exposure of sperm to shilajit. In the presence of various concentrations of shilajit, in vitro fertilization (IVF) of pig oocytes occurred with a reduced incubation time (3 hrs) and low sperm number (1 × 105 sperm·mL<SUP>-1</SUP>). The percentage of hyperactive sperm significantly increased with 10 and 20 μg·mL<SUP>-1</SUP> shilajit (p < 0.05). A significant number of intact acrosomal sperm were observed in sperm with 10 - 50 μg·mL<SUP>-1</SUP> shilajit (p < 0.05). In addition, higher adenosine triphosphate (ATP) content was observed in sperm exposed to these concentrations of shilajit compared to sperm incubated at 100 μg·mL<SUP>-1</SUP> shilajit or without shilajit (p < 0.05). When sperm were incubated with shilajit, the generation of ROS decreased compared to sperm without shilajit, and an increment of cytoplasmic Ca<SUP>2+</SUP> was observed in sperm exposed to 10 and 20 μg·mL<SUP>-1</SUP> shilajit (p < 0.05). In IVF trials, the sperm rapidly reached the oocytes in the presence of shilajit, and significantly higher total fertilization was observed in the IVF medium with 10 and 20 μg·mL<SUP>-1</SUP> shilajit. In conclusion, shilajit was seen to have a beneficial effect on improving sperm penetration and fertilization, suggesting that it could be a potential agent to improve fertilization rates in cases of subfertility and infertility during assisted reproduction.

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References

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