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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.7
- 수록면
- 247 - 258 (12page)
- DOI
- 10.1007/s11816-015-0361-8
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초록· 키워드
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This work examined the potential of Fourier transform infrared spectroscopy (FT-IR) spectroscopy and high-performance liquid chromatography (HPLC) analysis in evaluating metabolic changes during ripening of citrus fruit. Further, the feasibility of prediction modeling of carotenoid content by multivariate statistical analysis combined with FT-IR spectral and HPLC data was examined without additional HPLC analysis. FT-IR spectra of citrus (Citrus unshiu Marc. cv. Miyagawa) fruit peels and flesh were measured at monthly intervals of fruit development. Quantification of carotenoids from the fruit was confirmed by quantitative HPLC analysis. The most remarkable evolution of FT-IR spectral decrease during ripening of fruit was found in the amide region (1500?1700 cm-1), whereas there was an increase in the carbohydrate region (1000?1200 cm-1). The evolution of different FT-IR spectral bands was related to fruit constituents, including organic acids, carbohydrates, carotenoids, and phenolic compounds. Significant qualitative changes in the carotenoid pattern included an increase in bcryptoxanthin and decrease in lutein content during citrus fruit development. The content of antheraxanthin (R2 = 0.9117), β-carotene (R2 = 0.8816), β-cryptoxanthin (R2 = 0.8856), and violaxanthin (R2 = 0.7305) from peels of citrus fruit could be predicted from FT-IR spectral data using partial least square (PLS) regression modeling. Considering the results of PLS-discriminant analysis (PLSDA) of FT-IR spectral data and PLS regression modeling of carotenoid content, FT-IR in combination with multivariate analysis enables not only discrimination of metabolic variation during fruit development, but also prediction of carotenoid content from citrus fruit.
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참고문헌 (34)
참고문헌 신청
Armstrong GA / 1996 / Carotenoids 2 : genetics and molecular biology of carotenoid pigment biosynthesis / FASEB J 10:228~237
Baranska M / 2007 / Determination of lycopene and beta-carotene content in tomato fruits and related products: comparison of FT-Raman, ATR-IR, and NIR spectroscopy / Anal Chem 78:8456~8461
Baranski R / 2005 / Changes in carotenoid content and distribution in living plant tissue can be observed and mapped in situ using NIR-FT-Raman spectroscopy / Planta 222:448~457
Cerco´s M / 2006 / Globalanalysis of gene expression during development and ripening of citrus fruit flesh. A proposed mechanism for citric acid utilization / Plant Mol Biol 62:513~527
Cunningham FX / 1998 / Genes and enzymes of carotenoid biosynthesis in plants / Annu Rev Plant Physiol Plant Mol Biol 49:557~583
Baranska M / 2007 / Determination of lycopene and beta-carotene content in tomato fruits and related products: comparison of FT-Raman, ATR-IR, and NIR spectroscopy / Anal Chem 78:8456~8461
Baranski R / 2005 / Changes in carotenoid content and distribution in living plant tissue can be observed and mapped in situ using NIR-FT-Raman spectroscopy / Planta 222:448~457
Cerco´s M / 2006 / Globalanalysis of gene expression during development and ripening of citrus fruit flesh. A proposed mechanism for citric acid utilization / Plant Mol Biol 62:513~527
Cunningham FX / 1998 / Genes and enzymes of carotenoid biosynthesis in plants / Annu Rev Plant Physiol Plant Mol Biol 49:557~583
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