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자료유형
학술저널
저자정보
강은선 (전북대학교 원예학과) 김준수 (전북대학교) 은종선 (전북대학교)
저널정보
한국방사선산업학회 방사선산업학회지 방사선산업학회지 제8권 제2호
발행연도
2014.6
수록면
111 - 121 (11page)

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This study was aimed to select useful mutants of rapeseed (Brassica napus L.) and leafmustard (Brassica juncea L.), the seeds of three lines S-14, S-27, and S-28 were treated withgamma-ray and EMS. The optimum ranges of gamma-ray dose and EMS concentration toenlarge the characteristic morphological variations were also separately investigated. The survivalrates of S-28 only linearly decreased with increasing the gamma-ray dose. The overallgrowth parameters decreased of gamma-ray dose in all three lines of S-14, S-27, and S-28. Thereduction dosage 50 of gamma-ray was identified as 1,200 Gy for S-14 leaf mustard, while those ofS-27 and S-28 rapeseed lines were appeared as same 1,000 Gy. The emergence rates of S-14 andS-27 showed no significant differences by EMS treatment, while the growth of all three lines weresignificantly decreased. The reduction concentration 50 in S-14 could not be determined,demonstrating that this leaf mustard line is presumably insensitive to mutagenic EMS, whilethose in S-27 and S-28 were identified as 3.0 and 2.5%, respectively, showing that these rapeseedlines possess higher sensitivity to EMS than S-14. Various morphological characteristics of M1generation obtained from mutagen treatment were elaborately investigated for further maintenanceof M2 generation. In M2 generation variants showing short stem, yellow color in seed coat,chlorophyll deficiencies in leaf or pod, abnormal flower color were selected as potentially usefulmutants for breeding.

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