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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 113 - 118 (6page)
- DOI
- https://doi.org/10.14397/jals.2016.50.6.113
이용수
초록· 키워드
오류제보하기
Fast, cheap and sufficient serodiagnostic tools needs to be developed for the early detectionof brucellosis. Currently the tools cannot differentiate an active infection from vaccinated, norcan it differentiate other bacterial infections with lipopolysaccharides, especially Yersiniainfections. In this study, we purified recombinant outer membrane protein 10 and 28(rOmp10,rOmp28), and a dipstick assay(indirect or sandwich) was constructed with single(rOmp10 orrOmp28) and combined rOmps(rOmp10 and rOmp28) from Brucella(B.) abortus 544 to evaluatebovine Brucella positive serum collected during the beginning of the Korean outbreak from2006 to 2015. In application with single rOmp, rOmp10(70%; indirect, 92.11%; sandwichdipstick) and rOmp28(72.5%; indirect, 86.84%; sandwich dipstick) had comparable results. Inaddition, results indicated that dipstick with combined rOmps(rOmps10 and rOmp28) weresuperior in detecting positive serum samples, at 85% indirect and 100% sandwich dipstick.
Surprisingly, the results were the same in detecting negative results at 97.78% for both singleand combined indirect dipsticks. The dipstick tools with rOmp10 and rOmp28 would be usefulfor a rapid screen method for bovine brucellosis.
목차
등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Acha PN / 2003 / Zoonosis and communicable disease common to man and animals / Pan American Health Org :28~45
Clavijo E / 2003 / Comparison of a dipstick assay for detection of Brucella-specific immunoglobulin M antibodies with other tests for serodiagnosis of human brucellosis / Clin. Diagn. Lab. Immunol 10(4):612~615
Corbel MJ / 1997 / Recent advances in brucellosis / J. Med. Microbiol 46(2):101~103
Delpino MV / 2007 / Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB/c mice / Vaccine 25:6721~6729
Lee BY / 2006 / Surveillance and control of bovine brucellosis in the Republic of Korea during 2000-2006 / Prev. Vet. Med 90:66~79
Clavijo E / 2003 / Comparison of a dipstick assay for detection of Brucella-specific immunoglobulin M antibodies with other tests for serodiagnosis of human brucellosis / Clin. Diagn. Lab. Immunol 10(4):612~615
Corbel MJ / 1997 / Recent advances in brucellosis / J. Med. Microbiol 46(2):101~103
Delpino MV / 2007 / Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB/c mice / Vaccine 25:6721~6729
Lee BY / 2006 / Surveillance and control of bovine brucellosis in the Republic of Korea during 2000-2006 / Prev. Vet. Med 90:66~79
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