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논문 기본 정보

자료유형
학술저널
저자정보
신지은 (건국대학교 일반 대학원 생물공학과) 강상모 (건국대학교)
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한국미용학회 한국미용학회지 한국미용학회지 제19권 제1호
발행연도
2013.2
수록면
17 - 25 (9page)

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초록· 키워드

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This study was intended to clarify pre-treatment effect of the earthworm autolysates of which molecular weight distribution are different respectively in treating permanent wave on normal hair. We added the protease crude enzyme extract gotten by cultivating Bacillus subtilis K-54 separated from cheonggukjang (bean-paste soup prepared with ground fermented soybeans) when making the earthworm autolysates. In the meantime, we made the molecular weight distribution of earthworm autolysates became different through making the additive amount of protease crude enzyme became different. We observed the molecular weight distribution of earthworm autolysates using SDS-PAGE, and measured hair thickness, tensile strength, density analysis by methylene blue dyeing, wave formation and maintenance strength with the protease crude enzyme (0.112 unit) after carrying out the pre-treatment on each autolysates. In the event of making the earthworm autolysates, the highest rate for the permanent effect was shown at LbB50(0.112+0.0895×50 unit) of which protease per 1 g earthworm was added by about 30 times. As the result of study, for securing quality as a pre-treatment agent in treating permanent wave on normal hair, it was thought that it is more important to make the earthworm protein became the decompression substances with the proper size than to make them became the low molecular protein.

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