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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제18권 제4호
- 발행연도
- 2014.8
- 수록면
- 297 - 305 (9page)
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Flavonoids have an ability to suppress various ion channels. We determined whether one offlavonoids, cyanidin-3-glucoside, affects adenosine 5'-triphosphate (ATP)-induced calcium signalingusing digital imaging methods for intracellular free Ca2＋ concentration ([Ca2＋]i), reactive oxygenspecies (ROS) and mitochondrial membrane potential in PC12 cells. Treatment with ATP (100μM)for 90 sec induced [Ca2＋]i increases in PC12 cells. Pretreatment with cyanidin-3-glucoside (1μ g/mlto 100μg/ml) for 30 min inhibited the ATP-induced [Ca2＋]i increases in a concentration-dependentmanner (IC50=15.3μg/ml). Pretreatment with cyanidin-3-glucoside (15μg/ml) for 30 min significantlyinhibited the ATP-induced [Ca2＋]i responses following removal of extracellular Ca2＋ or depletion ofintracellular [Ca2＋]i stores. Cyanidin-3-glucoside also significantly inhibited the relatively specific P2X2receptor agonist 2-MeSATP-induced [Ca2＋]i responses. Cyanidin-3-glucoside significantly inhibited thethapsigargin or ATP-induced store-operated calcium entry. Cyanidin-3-glucoside significantly inhibitedthe ATP-induced [Ca2＋]i responses in the presence of nimodipine and ω -conotoxin. Cyanidin-3-glucoside also significantly inhibited KCl (50 mM)-induced [Ca2＋]i increases. Cyanidin-3-glucosidesignificantly inhibited ATP-induced mitochondrial depolarization. The intracellular Ca2＋ chelatorBAPTA-AM or the mitochondrial Ca2＋ uniporter inhibitor RU360 blocked the ATP-induced mitochondrialdepolarization in the presence of cyanidin-3-glucoside. Cyanidin-3-glucoside blocked ATPinducedformation of ROS. BAPTA-AM further decreased the formation of ROS in the presence ofcyanidin-3-glucoside. All these results suggest that cyanidin-3-glucoside inhibits ATP-induced calciumsignaling in PC12 cells by inhibiting multiple pathways which are the influx of extracellular Ca2＋through the nimodipine and ω -conotoxin-sensitive and -insensitive pathways and the release of Ca2＋from intracellular stores. In addition, cyanidin-3-glucoside inhibits ATP-induced formation of ROS byinhibiting Ca2＋-induced mitochondrial depolarization.
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참고문헌 (39)
참고문헌 신청
Kim HJ / 2005 / Fluoxetine inhibits ATP-induced [Ca2+]i increase in PC12 cells by inhibiting both extracellular Ca2+ influx and Ca2+ release from intracellular stores / Neuropharmacology 49:265~274
Nasr P / 2003 / Influence of cytosolic and mitochondrial Ca2+, ATP, mitochondrial membrane potential, and calpain activity on the mechanism of neuron death induced by 3-nitropropionic acid / Neurochem Int 43:89~99
Brookes PS / 2004 / Calcium, ATP, and ROS: a mitochondrial love-hate triangle / Am J Physiol Cell Physiol 287:C817~C833
Sun AY / 1998 / Extracellular ATP-induced apoptosis in PC12 cells / Adv Exp Med Biol 446:73~83
Vollmayer P / 2001 / Multiple ecto-nucleotidases in PC12cells: identification and cellular distribution after heterologous expression / J Neurochem 78:1019~1028
Nasr P / 2003 / Influence of cytosolic and mitochondrial Ca2+, ATP, mitochondrial membrane potential, and calpain activity on the mechanism of neuron death induced by 3-nitropropionic acid / Neurochem Int 43:89~99
Brookes PS / 2004 / Calcium, ATP, and ROS: a mitochondrial love-hate triangle / Am J Physiol Cell Physiol 287:C817~C833
Sun AY / 1998 / Extracellular ATP-induced apoptosis in PC12 cells / Adv Exp Med Biol 446:73~83
Vollmayer P / 2001 / Multiple ecto-nucleotidases in PC12cells: identification and cellular distribution after heterologous expression / J Neurochem 78:1019~1028
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