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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.7
- 수록면
- 630 - 637 (8page)
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초록· 키워드
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Morus alba root extract (MARE) has been used to treat hyperglycaemic conditions in oriental medicine. Here, we studied whether MARE possesses a cytotoxic effect on neuroblastoma. To check the cytotoxicity generated by MARE was whether relatively higher against the cancer cells rather than normal cells, we chose a neuroblastoma cell line (B103) and a normal cell line (Rat-2). A CCK assay revealed that MARE (10μg/ml) reduced cell viability to approximately 60% compared to an untreated control in B103 cells. But in Rat-2 cells, MARE induced relatively lower cytotoxicity. To investigate the mechanisms underlying the cytotoxic effect of MARE, we used flow cytometry combined with immunoblot analyses. We found that MARE-treatment could accumulate ROS and depolarize mitochondria membrane potential of B103 cells. Further treatment with MARE in B103 cells also could damage DNA and induce apoptosis. An expression study of p-Akt also suggested that there was a reduction in cellular proliferation and transcription along with the process of apoptosis, which was further evidenced by an increase in Bax and cleaved-caspase 3 activity. Together, our findings suggest that MARE produces more cytotoxicity in cancer cells while having a relatively attenuated effect on normal cells. As such, MARE may be a safer option in cancer therapeutics, and it also shows potential for the patients with symptoms of hyperglycemia and cancer.
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참고문헌 (36)
참고문헌 신청
Bengmark, S. / 2009 / Plant-derived health : the effects of turmeric and curcuminoids / Nutr. Hosp 24:273~281
Boreddy, S. R. / 2011 / Pancreatic tumor suppression by benzyl isothiocyanate is associated with inhibition of PI3K/AKT/FOXO pathway / Clin. Cancer Res 17:1784~1795
Brunet, A. / 1999 / Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor / Cell 96:857~868
Carpenter, C. L. / 1990 / Purification and characterization of phosphoinositide 3-kinase from rat liver / J. Biol. Chem 265:19704~19711
Chakraborty, D. / 2013 / Antihyperglycemic drug Gymnema sylvestre also shows anticancer potentials in human melanoma A375 cells via reactive oxygen species generation and mitochondria-dependent caspase pathway / Integr. Cancer Ther 12:433~441
Boreddy, S. R. / 2011 / Pancreatic tumor suppression by benzyl isothiocyanate is associated with inhibition of PI3K/AKT/FOXO pathway / Clin. Cancer Res 17:1784~1795
Brunet, A. / 1999 / Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor / Cell 96:857~868
Carpenter, C. L. / 1990 / Purification and characterization of phosphoinositide 3-kinase from rat liver / J. Biol. Chem 265:19704~19711
Chakraborty, D. / 2013 / Antihyperglycemic drug Gymnema sylvestre also shows anticancer potentials in human melanoma A375 cells via reactive oxygen species generation and mitochondria-dependent caspase pathway / Integr. Cancer Ther 12:433~441
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