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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 81 - 88 (8page)
- DOI
- 10.14348/molcells.2015.2254
이용수
초록· 키워드
오류제보하기
Flowering time (or heading date) is controlled by intrinsic genetic programs in response to environmental cues, such as photoperiod and temperature. Rice, a facultative short-day (SD) plant, flowers early in SD and late in long-day (LD) conditions. Casein kinases (CKs) generally act as positive regulators in many signaling pathways in plants. In rice, Heading date 6 (Hd6) and Hd16 encode CK2α and CKI, respectively, and mainly function to delay flowering time. Additionally, the major LD-dependent floral repressors Hd2/Oryza sativa Pseudo-Response Regulator 37 (OsPRR37; hereafter PRR37) and Ghd7 also confer strong photoperiod sensitivity. In floral induction, Hd16 acts upstream of Ghd7 and CKI interacts with and phosphorylates Ghd7. In addition, Hd6 and Hd16 also act upstream of Hd2. However, whether CKI and CK2α directly regulate the function of PRR37 remains unclear. Here, we use in vitro pull-down and in vivo bimolecular fluorescence complementation assays to show that CKI and CK2α interact with PRR37. We further use in vitro kinase assays to show that CKI and CK2α phosphorylate different regions of PRR37. Our results indicate that direct posttranslational modification of PRR37 mediates the genetic interactions between these two protein kinases and PRR37. The significance of CK-mediated phosphorylation for PRR37 and Ghd7 function is discussed.
목차
등록된 정보가 없습니다.
참고문헌 (74)
참고문헌 신청
Abe, M. / 2005 / FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex / Science 309:1052~1056
Alabadi, D. / 2001 / Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock / Science 293:880~883
Beales, J. / 2007 / A pseudo-response regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat(Triticum aestivum L. ) / Theor. Appl. Genet 115:721~733
Citovsky, V. / 2006 / Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta / J. Mol. Biol 362:1120~1131
Corbesier, L. / 2007 / FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis / Science 316:1030~1033
Alabadi, D. / 2001 / Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock / Science 293:880~883
Beales, J. / 2007 / A pseudo-response regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat(Triticum aestivum L. ) / Theor. Appl. Genet 115:721~733
Citovsky, V. / 2006 / Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta / J. Mol. Biol 362:1120~1131
Corbesier, L. / 2007 / FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis / Science 316:1030~1033
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