지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.3
- 수록면
- 257 - 263 (7page)
- DOI
- 10.14348/molcells.2014.2384
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초록· 키워드
오류제보하기
A mammalian cell renovates itself by autophagy, a pro-cess through which cellular components are recycled to produce energy and maintain homeostasis. Recently, the abundance of gap junction proteins was shown to be regulated by autophagy during starvation conditions, suggesting that transmembrane proteins are also regulated by autophagy. Transient receptor potential vanilloid type 1 (TRPV1), an ion channel loca-lized to the plasma membrane and endoplasmic reticu-lum (ER), is a sensory transducer that is activated by a wide variety of exogenous and endogenous physical and chemical stimuli. Intriguingly, the abundance of cellular TRPV1 can change dynamically under pathological conditions. However, the mechanisms by which the protein levels of TRPV1 are regulated have not yet been explored. Therefore, we investigated the mechanisms of TRPV1 recycling using HeLa cells constitutively expressing TRPV1. Endogenous TRPV1 was degra-ded in starvation conditions; this degradation was blocked by chloroquine (CLQ), 3MA, or downregulation of Atg7. Interestingly, a glucocorticoid (cortisol) was capable of inducing autophagy in HeLa cells. Cortisol increased cellular conversion of LC3-I to LC-3II, leading autophagy and resulting in TRPV1 degradation, which was similarly inhibited by treatment with CLQ, 3MA, or downre-gulation of Atg7. Furthermore, cortisol treatment induced the colocalization of GFP-LC3 with endogenous TRPV1. Cumulatively, these observations provide evidence that degradation of TRPV1 is mediated by autophagy, and that this pathway can be enhanced by cortisol.
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참고문헌 (34)
참고문헌 신청
Ahn, S. / 2013 / CK2-mediated TEL2 phosphorylation augments nonsense-mediated mRNA decay(NMD)by increase of SMG1 stability / Biochim. Biophys. Acta 1829:1047~1055
Bejarano, E. / 2012 / Autophagy modulates dynamics of connexins at the plasma membrane in a ubiquitin-dependent manner / Mol. Biol. Cell 23:2156~2169
Biggs, J. E. / 2007 / Changes in vanilloid receptor 1(TRPV1)expression following lingual nerve injury / Eur. J. Pain 11:192~201
Caterina, M. J. / 1997 / The capsaicin receptor : a heat-activated ion channel in the pain pathway / Nature 389:816~824
Clapham, D. E. / 2003 / TRP channels as cellular sensors / Nature 426:517~524
Bejarano, E. / 2012 / Autophagy modulates dynamics of connexins at the plasma membrane in a ubiquitin-dependent manner / Mol. Biol. Cell 23:2156~2169
Biggs, J. E. / 2007 / Changes in vanilloid receptor 1(TRPV1)expression following lingual nerve injury / Eur. J. Pain 11:192~201
Caterina, M. J. / 1997 / The capsaicin receptor : a heat-activated ion channel in the pain pathway / Nature 389:816~824
Clapham, D. E. / 2003 / TRP channels as cellular sensors / Nature 426:517~524
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