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자료유형
학술저널
저자정보
Zhu Yanyan (Anhui Agricultural University) Tang Wei (Anhui Agricultural University) Tang Xiaofeng (Hefei University of Technology) Wang Lihuan (Anhui Agricultural University) Li Wei (Anhui Agricultural University) Zhang Qian (Sichuan Academy of Natural Resource Sciences) Li Mingzhang (Sichuan Academy of Natural Resource Sciences) Fang Congbing (Anhui Agricultural University) Liu Yongsheng (Anhui Agricultural University) Wang Songhu (Anhui Agricultural University)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제15권 제5호
발행연도
2021.10
수록면
673 - 682 (10page)
DOI
10.1007/s11816-021-00704-2

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Colchicine-induced polyploidization has been extensively utilized in plant-breeding programs to increase biomass and overall yield of various crop species. Chromosome doubling usually increases the plant size and cell size. However, the underly- ing mechanisms remain elusive. In this study, we showed that 0.1% colchicine is an optimized concentration for inducing tetraploidization of Actinidia chinensis var. chinensis ‘Hongyang’, a commercially important diploid kiwifruit cultivar. The tetraploid plants showed increased plant height, leaf size, and biomass, as compared with the corresponding diploid plants. Scanning electron microscopy and histological analysis indicated that the leaf cell size was significantly increased in the tetraploid plants. Our further transcriptome analysis revealed the 5922 differentially expressed genes between the diploid and tetraploid plants. Gene Ontology analysis enriched the cell wall-related genes, including the pectin methylesterases (PMEs) and expansins (EXPs), both of which play a critical role in cell wall loosening and extension. The increased expression of PME and EXP genes might contribute to the increased cell size in the tetraploid plants. Together, our work indicated that tetraploidization increases the organ and cell size in kiwifruit, possibly by enhancing the cell wall extensibility.

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