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논문 기본 정보

자료유형
학술저널
저자정보
Tien‑Hsin Chuang (National Chung Hsing University) Kun‑Hung Li (National Chung Hsing University) Pei‑Fang Li (National Chung Hsing University) Chang‑Hsien Yang (National Chung Hsing University)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제12권 제2호
발행연도
2018.4
수록면
115 - 125 (11page)

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An Eustoma grandiflorum APETALA1 (EgAP1) gene showing high homology to the SQUA subfamily of MADS-box genes was isolated and characterized. EgAP1, containing a conserved euAP1 motif at the C-terminus, showed high sequence identity to Antirrhinum majus SQUAMOSA in the SQUA subfamily. EgAP1 mRNA was detected in the leaf and expressed significantly higher in young flower buds than in mature flower buds. In flowers, EgAP1 mRNA was strongly detected in sepal, weakly detected in petal and was absent in stamen and carpel. Transgenic Arabidopsis plants ectopically expressing EgAP1 flowered early and produced terminal flowers. In addition, the conversion of petals into stamen-like structures was also observed in 35S::EgAP1 flowers. 35S::EgAP1 was able to complement the ap1 flower defects by restoring the defect for sepal formation and significantly increasing second whorl petal production in Arabidopsis ap1 mutant plants. These results revealed that EgAP1 is the APETALA1 homolog in E. grandiflorum and that the function of EgAP1 is involved in floral induction and flower formation.

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