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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.8
- 수록면
- 239 - 245 (7page)
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Class-I patatin genes, which encode the potato storage protein patatin, are tuber speci?c in their expression, and several promoter elements directing the tissuespeci?c expression of the patatin genes have been known for long time. However, the identity of the relevant transcription factors is still unknown. In this study, we show that K box, one of the protein-binding sites in a class-I patatin gene promoter, is a novel cis-regulatory element required for the tuber-speci?c expression of the gene. Subsequently, we isolated K-box-binding factors by yeast one-hybrid screening. Analysis of the factors revealed that they are MYB class transcription factors, which we named TSF1?4 (tuber-speci?c and sucrose-inducible elementbinding factors 1?4). TSFs possess transcriptional activity to various degrees, and overexpression of TSF2, which possesses the highest transcriptional activity, induced the ectopic expression of patatin genes in potato leaves. Our results strongly suggest that TSFs could play regulatory function in the expression of patatin genes in planta.
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참고문헌 (28)
참고문헌 신청
Andrews DL / 1988 / Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector / Biochem J 252:199~206
Bevan M / 1986 / The structure and transcription start site of a major potato tuber protein gene / Nucleic Acids Res 14:4625~4638
Choi H / 2000 / ABFs, a family of ABA-responsive element binding factors / J Biol Chem 275:1723~1730
Chomczynski P / 1995 / Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources / Biotechniques 19:942~945
Fu H / 1995 / High-level tuber expression and sucrose inducibility of a potato Sus4 sucrose synthase gene require 50 and 30 flanking sequences and the leader intron / Plant Cell 7:1387~1394
Bevan M / 1986 / The structure and transcription start site of a major potato tuber protein gene / Nucleic Acids Res 14:4625~4638
Choi H / 2000 / ABFs, a family of ABA-responsive element binding factors / J Biol Chem 275:1723~1730
Chomczynski P / 1995 / Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources / Biotechniques 19:942~945
Fu H / 1995 / High-level tuber expression and sucrose inducibility of a potato Sus4 sucrose synthase gene require 50 and 30 flanking sequences and the leader intron / Plant Cell 7:1387~1394
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