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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.4
- 수록면
- 127 - 133 (7page)
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SNPs can be used to discriminate allele-specific genotypes based on the 30-terminal nucleotide of a primer corresponding to a specific SNP site. However, reliable discrimination between alleles in closely related species is not sufficient for molecular genetic identification. To overcome this problem, the present study evaluated the amplification refractory mutation system (ARMS)-PCR analysis method to discriminate closely related species, which utilizes specific primers that have a base pair change within three bases closest to the SNP site. The aim of this study was to develop a simple and precise DNA-based method for molecular authentication of C. wilfordii when compared with the highly similar species C. auriculatum.
Molecular authentication of C. wilfordii and C. auriculatum was achieved using specific modified primers for DNA sequences of chloroplast TrnL-F and nuclear internal transcribed spacer (ITS) regions. A species-specific SNP was detected for each of the two species, and mismatched ARMS-PCR was conducted to specifically identify the SNP site. Plant samples collected from different locations were used to validate the selectively modified SNP markers, and the established method was determined to be effective. Therefore, this study provides a rapid and reliable method for specific identification of the medicinal plants, C. wilfordii and C. auriculatum.
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등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
Cha RS / 1992 / Mismatch amplification mutation assay (MAMA): application to the c-Hras gene / Genome Res 2:14~20
Han EH / 2016 / Development of molecular markers, based on chloroplast and ribosomal DNA regions, to discriminate three popular medicinal plant species, Cynanchum wilfordii, Cynanchum auriculatum, and Polygonum multiflorum / Mol Biol Rep 43:323~332
Hayashi K / 2004 / Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus / Theor Appl Genet 108:1212~1220
Hirotsu N / 2010 / Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species / Plant Methods 6:12
김현순 / 2016 / Comparative transcriptome profiling and SSR marker identification in three Jerusalem artichoke (Helianthus tuberosus L.) cultivars exhibiting phenotypic variation / Plant Biotechnology Reports 10(6):447~461
Han EH / 2016 / Development of molecular markers, based on chloroplast and ribosomal DNA regions, to discriminate three popular medicinal plant species, Cynanchum wilfordii, Cynanchum auriculatum, and Polygonum multiflorum / Mol Biol Rep 43:323~332
Hayashi K / 2004 / Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus / Theor Appl Genet 108:1212~1220
Hirotsu N / 2010 / Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species / Plant Methods 6:12
김현순 / 2016 / Comparative transcriptome profiling and SSR marker identification in three Jerusalem artichoke (Helianthus tuberosus L.) cultivars exhibiting phenotypic variation / Plant Biotechnology Reports 10(6):447~461
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