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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 강원대학교 산림과학연구소 Journal of forest and environmental science Journal of forest and environmental science 제33권 제3호
- 발행연도
- 2017.8
- 수록면
- 197 - 201 (5page)
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초록· 키워드
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Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at α?=0.01%. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at α=0.05%. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Ahmedi A / 2008 / Embryo-rescue as a tool in Inter generic in Salix famiy(Salix alba × Populus caspica)hybridization / Iranian Journal of Rangelands and Forestes Plant Breedling and Genetic Research 6:149~157
Ahuja MR. / 1986 / In ‘Handbook of plant cell culture. Vol. 4. Techniques and pplications / WR Sharp :626~651
Bagniewska Zadworna A / 2011 / A successful application of the embryo rescue technique as a model for studying crosses between Salix viminalis and Populus species / Aust J Bot 59:382~392
Calagari M / 2003 / Interspecific hybridization of P. euphratica Olive. Through embryo rescue / Pajoohesh & Sazandegi 61:6~9
Fernando DD / 2005 / Sexual reproduction and crossing barriers in white pines : the case between Pinus lambertiana(sugar pine)and P. monticola(western white pine) / Tree Genetics & Genomes 1:143~150
Ahuja MR. / 1986 / In ‘Handbook of plant cell culture. Vol. 4. Techniques and pplications / WR Sharp :626~651
Bagniewska Zadworna A / 2011 / A successful application of the embryo rescue technique as a model for studying crosses between Salix viminalis and Populus species / Aust J Bot 59:382~392
Calagari M / 2003 / Interspecific hybridization of P. euphratica Olive. Through embryo rescue / Pajoohesh & Sazandegi 61:6~9
Fernando DD / 2005 / Sexual reproduction and crossing barriers in white pines : the case between Pinus lambertiana(sugar pine)and P. monticola(western white pine) / Tree Genetics & Genomes 1:143~150
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