지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 313 - 321 (9page)
- DOI
- 10.12997/jla.2021.10.3.313
이용수
초록· 키워드
오류제보하기
Objective: The liver plays a central role in lipid metabolism. During fasting and feeding, the fatty acid trafficking between adipose tissue and liver induces accumulation and dissociation of dynamic hepatic lipid droplets (LDs). Herein, we established an intravital 2-photon imaging technique to longitudinally visualize the dynamic in vivo alteration of hepatic LD deposition during fasting and refeeding in the liver of live mouse.
Methods: Intravital 2-photon imaging of liver was performed to observe hepatic LD alteration induced by fasting for different periods of time, 12, 24, and 48 hours followed by refeeding.
Hepatic LDs were fluorescently labelled in vivo by intravenous injection of Seoul-Flour 44 and visualized by custom-built intravital 2-photon microscope.
Results: Significant increases of the number and size of hepatic LDs were observed by intravital 2-photon imaging of the liver after 12 hours of fasting. The degree of hepatic LD accumulation continuously increased with fasting up to 48 hours. Remarkably, with refeeding for 24 hours, the hepatic LDs accumulated by fasting were fully dissociated and the LD occupancy in the liver was recovered to the normal state.
Conclusion: Utilizing intravital 2-photon microscope with in vivo systemic fluorescent labeling of LD in live mice, dynamic alterations of hepatic LDs such as accumulation and dissociation by fasting and refeeding were successfully visualized at a subcellular level in vivo. The established method enabling the in vivo visualization of LDs will be a useful tool to investigate the pathophysiology of various diseases associated with dysregulated lipid metabolism.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Nguyen P / 2008 / Liver lipid metabolism / J Anim Physiol Anim Nutr(Berl) 92:272~283
Cahill GF Jr / 2006 / Fuel metabolism in starvation / Annu Rev Nutr 26:1~22
Hashimoto T / 2000 / Defect in peroxisome proliferator-activated receptor α-inducible fatty acid oxidation determines the severity of hepatic steatosis in response to fasting / J Biol Chem 275:28918~28928
Guan HP / 2009 / Accelerated fatty acid oxidation in muscle averts fastinginduced hepatic steatosis in SJL/J mice / J Biol Chem 284:24644~24652
Chitraju C / 2012 / Lipidomic analysis of lipid droplets from murine hepatocytes reveals distinct signatures for nutritional stress / J Lipid Res 53:2141~2152
Cahill GF Jr / 2006 / Fuel metabolism in starvation / Annu Rev Nutr 26:1~22
Hashimoto T / 2000 / Defect in peroxisome proliferator-activated receptor α-inducible fatty acid oxidation determines the severity of hepatic steatosis in response to fasting / J Biol Chem 275:28918~28928
Guan HP / 2009 / Accelerated fatty acid oxidation in muscle averts fastinginduced hepatic steatosis in SJL/J mice / J Biol Chem 284:24644~24652
Chitraju C / 2012 / Lipidomic analysis of lipid droplets from murine hepatocytes reveals distinct signatures for nutritional stress / J Lipid Res 53:2141~2152
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Intravital confocal & two-photon microscopy for in vivo cellular-level imaging of live animal model
한국실험동물학회 학술발표대회 논문집
2025 .02
-
In vivo visualization of cellular dynamics in living animal
한국실험동물학회 학술발표대회 논문집
2025 .02
-
Intravital Laser-scanning Two-photon and Confocal Microscopy for Biomedical Research
MEDICAL LASERS
2021 .01
-
Intravital confocal and two-photon microscopy for in vivo live cell imaging in small animal model
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Two-photon intravital imaging of leukocyte migration during inflammation in the respiratory system
Acute and Critical Care
2019 .01
-
LD Flap Reconstruction
대한외과학회 학술대회 초록집
2015 .11
-
Intravital microscopy in cancer immunology
대한임상종양학회 학술대회지
2019 .02
-
Intravital imaging set up of neutrophil extracellular trap in mouse brain to investigate the role of neutrophils in neuroinflammatory diseases
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Neutrophil Extravasation Cascade: What Can We Learn from Two-photon Intravital Imaging?
Immune Network
2016 .12
-
Real-time dynamics of cancer cell recognition by innate immune cells in live mouse bone marrow
한국실험동물학회 학술발표대회 논문집
2019 .07
-
The Effectiveness of Lumbar Cerebrospinal Fluid Drainage to Reduce the Cerebral Vasospasm after Surgical Clipping for Aneurysmal Subarachnoid Hemorrhage
대한신경외과학회지
2015 .01
-
Mini LD Flap Replacement
대한외과학회 학술대회 초록집
2015 .11
-
사염화탄소 유도성 급성 간 손상 모델에서 레몬밤과 민들레 잎 추출물의 혼합 비율에 따른 간 보호 효능 연구
대한한의학방제학회지
2019 .01
-
Pathogenesis and treatment of non-alcoholic steatohepatitis and its fibrosis
Clinical and Molecular Hepatology
2023 .01
-
언어발달지체 영유아의 한국판 LDS(언어발달검사) 반응특성
한국놀이치료학회지
2016 .01
-
Perilipin 5 is regulated by LRH-1 and is a key mediator of hepatic triglyceride metabolism in the fasting liver
한국실험동물학회 학술발표대회 논문집
2022 .01
-
Changes in the types of liver diseases requiring hepatic resection : A single-institution experience of 9016 cases over a 10-year period
한국간담췌외과학회 학술대회지
2016 .04
-
Percutaneous transhepatic hepatic venous stenting after extracorporeal hepatic resection and autotransplantation: A case report
International Journal of Gastrointestinal Intervention
2017 .10
-
Evaluation and Management of Hepatic Encephalopathy: Current Status and Future Directions
Gut and Liver
2016 .01
-
Fatal neurological complication after liver transplantation in acute hepatic failure patient with hepatic encephalopathy
고신대학교 의과대학 학술지
2018 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0