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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 326 - 336 (11page)
- DOI
- 10.12701/yujm.2021.01025
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초록· 키워드
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Background: Sulfation of heparan sulfate proteoglycans (HSPGs) is critical for the binding and signaling of ligands that mediate inflammation. Extracellular 6-O-endosulfatases regulate posttranslational sulfation levels and patterns of HSPGs. In this study, extracellular 6-O-endosulfatases, sulfatase (Sulf)-1 and Sulf-2, were evaluated for their expression and function in inflammatory cells and tissues. Methods: Harvested human peripheral blood mononuclear cells were treated with phytohemagglutinin and lipopolysaccharide, and murine peritoneal macrophages were stimulated with interleukin (IL)-1β for the evaluation of Sulf-1 and Sulf-2 expression. Sulf expression in inflammatory cells was examined in the human rheumatoid arthritis (RA) synovium by immunofluorescence staining. The antigen presentation and phagocytic activities of macrophages were compared according to the expression state of Sulfs. Sulfs-knockdown macrophages and Sulfs-overexpressing macrophages were generated using small interfering RNAs and pcDNA3.1 plasmids for Sulf-1 and Sulf-2, respectively. Results: Lymphocytes and monocytes showed weak Sulf expression, which remained unaffected by IL-1β. However, peritoneal macrophages showed increased expression of Sulfs upon stimulation with IL-1β. In human RA synovium, two-colored double immunofluorescent staining of Sulfs and CD68 revealed active upregulation of Sulfs in macrophages of inflamed tissues, but not in lymphocytes of lymphoid follicles. Macrophages are professional antigen-presenting cells. The antigen presentation and phagocytic activities of macrophages were dependent on the level of Sulf expression, suppressed in Sulfs-knockdown macrophages, and enhanced in Sulfs-overexpressing macrophages. Conclusion: The results demonstrate that upregulation of Sulfs in macrophages occurs in response to inflammation, and Sulfs actively regulate the antigen presentation and phagocytic activities of macrophages as novel immune regulators.
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참고문헌 (40)
참고문헌 신청
Dreyfuss JL / 2009 / Heparan sulfate proteoglycans: structure, protein interactions and cell signaling / An Acad Bras Cienc 81:409~429
Clasper S / 1999 / Inducible expression of the cell surface heparan sulfate proteoglycan syndecan-2(fibroglycan)on human activated macrophages can regulate fibroblast growth factor action / J Biol Chem 274:24113~24123
Parish CR / 2006 / The role of heparan sulphate in inflammation / Nat Rev Immunol 6:633~643
Vanpouille C / 2007 / The heparin/heparan sulfate sequence that interacts with cyclophilin B contains a 3-O-sulfated N-unsubstituted glucosamine residue / J Biol Chem 282:24416~24429
Matsuo I / 2013 / Extracellular modulation of fibroblast growth factor signaling through heparan sulfate proteoglycans in mammalian development / Curr Opin Genet Dev 23:399~407
Clasper S / 1999 / Inducible expression of the cell surface heparan sulfate proteoglycan syndecan-2(fibroglycan)on human activated macrophages can regulate fibroblast growth factor action / J Biol Chem 274:24113~24123
Parish CR / 2006 / The role of heparan sulphate in inflammation / Nat Rev Immunol 6:633~643
Vanpouille C / 2007 / The heparin/heparan sulfate sequence that interacts with cyclophilin B contains a 3-O-sulfated N-unsubstituted glucosamine residue / J Biol Chem 282:24416~24429
Matsuo I / 2013 / Extracellular modulation of fibroblast growth factor signaling through heparan sulfate proteoglycans in mammalian development / Curr Opin Genet Dev 23:399~407
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