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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 305 - 311 (7page)
- DOI
- 10.1007/s13206-021-00034-z
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초록· 키워드
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The outbreak of new viral strains promotes advances in universal diagnostic techniques for detecting infectious diseases with unknown viral sequence. Long double-stranded RNA (dsRNA), a hallmark of infections, serves as a virus marker for prompt detection of viruses with unknown genomes. Here, we report on-chip paper electrophoresis for ultrafast screening of infectious diseases. Negatively charged RNAs pass through the micro and nanoscale pores of cellulose in order of size under an external electric field applied to the paper microfluidic channel. Quantitative separation of long dsRNA mimicking poly I:C was analyzed from 1.67 to 33 ng·μL ?1 , which is close to the viral dsRNA concentration in infected cells. This paper-based capillary electrophoresis chip (paper CE chip) can provide a new diagnostic platform for ultrafast viral disease detection at the point-of-care (POC) level.
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참고문헌 (39)
참고문헌 신청
Jasper Fuk-Woo Chan / 2020 / Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens / Journal of Clinical Microbiology 58(5)
Castro, A.C.H. / 2014 / Preparation of genosensor for detection of specifi c DNA sequence of the hepatitis B virus / Appl. Surf. Sci 314:273~279
Kim, D. / 2020 / The architecture of SARS-CoV-2 transcriptome / Cell 181:914~921.e10
Monsion, B. / 2018 / Effi cient detection of long dsRNA in vitro and in vivo using the dsRNA binding domain from FHV B2 protein / Front. Plant Sci 9:70
Jayoung Ku / 2020 / Reactive Polymer Targeting dsRNA as Universal Virus Detection Platform with Enhanced Sensitivity / Biomacromolecules 21(6):2440~2454
Castro, A.C.H. / 2014 / Preparation of genosensor for detection of specifi c DNA sequence of the hepatitis B virus / Appl. Surf. Sci 314:273~279
Kim, D. / 2020 / The architecture of SARS-CoV-2 transcriptome / Cell 181:914~921.e10
Monsion, B. / 2018 / Effi cient detection of long dsRNA in vitro and in vivo using the dsRNA binding domain from FHV B2 protein / Front. Plant Sci 9:70
Jayoung Ku / 2020 / Reactive Polymer Targeting dsRNA as Universal Virus Detection Platform with Enhanced Sensitivity / Biomacromolecules 21(6):2440~2454
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