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논문 기본 정보

자료유형
학술저널
저자정보
Jeong-Hee Yu (Department of Veterinary Public Health College of Veterinary Medicine Jeonbuk National University) Yeong-Ju Yu (Department of Veterinary Public Health College of Veterinary Medicine Jeonbuk National University) Seon-Min Kim (Department of Veterinary Public Health College of Veterinary Medicine Jeonbuk National University) Jin Hur (Department of Veterinary Public Health College of Veterinary Medicine Jeonbuk National University)
저널정보
한국동물위생학회(구 한국가축위생학회) 한국가축위생학회지 한국가축위생학회지 제44권 제4호
발행연도
2021.12
수록면
247 - 256 (10page)
DOI
10.7853/kjvs.2021.44.4.247

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초록· 키워드

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The recombinant lysozyme-HJL34 proteins were expressed and purified using commercial Esch-erichia (E .) coli expression system. Stx2e+ F18+ E. coli , Actinobacillus pleuropneumoniae (APP ), Streptococcus (S .) suis , and Clostridium (C .) perfringens strains were isolated from pigs. The mini-mum inhibitory concentrations (MICs) of the recombinant lysozyme-HJP34 proteins were examined by means of the microtiter plate method, according to the NCCLS recommendations. The possibility of its as the alternatives to antibiotics was tested in piglets. The MICs were determined as 75 mg/mL, 300 mg/mL, 75 mg/mL, 35.5 mg/m against Stx2e+ F18+ E. coli , APP, S. suis , C. perfringens , respectively. A total of 25 piglets were divied 5 groups. The piglets in group A∼C were fed with commercial feed and those in groups D, E were fed with commercial feedstuff. All piglets in groups B∼E were chal-lenged with virulent Stx2e+ F18+ E. coli , APP, S. suis strains. Groups C and D were treated with anti-microbial from 24 h after challenge. All piglets in group B died within 3 days after challenge. Among 5 piglets in groups C and D piglets, 80% survived after challenge. Among group E piglets, 60% were alive until the end of this study. Therefore, this study indicates that recombinant lysozyme-HJP34 proteins is a suitable possibility as a feed additive for reduction of diseases by bacterial pathogens in piglet feed.

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