지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 65 - 73 (9page)
- DOI
- https://doi.org/10.14397/jals.2021.55.6.65
이용수
초록· 키워드
오류제보하기
In the present study, we examined the effect of different lecithin concentrations on spermatozoa characteristics after freeze-thawing.
Hanwoo semen was collected from one bull and divided into five groups (tris-citric acid semen extender with 0, 0.1, 0.25, and 0.5% lecithin groups as well as a 20% egg yolk group). Semen extender with 20% egg yolk was used as control treatment. After the freeze-thawing of semen, spermatozoa motility, motility parameters, viability, acrosomal membrane integrity, mitochondrial membrane potential, and plasma membrane integrity were examined. In experiment 1, the effect of different lecithin concentrations on spermatozoa motility and associated parameters was examined. The 0.1% lecithin-treated spermatozoa showed greater fast progressive motility (%) in addition to higher VCL (μm/s), VSL (μm/s), and VAP (μm/s) when compared to other lecithin concentration groups and controls. In experiment 2, the effect of different lecithin concentrations on spermatozoa viability was examined. The 0.1% and 0.25% lecithin addition groups (55.4±7.3 and 51.7±11.2%) exhibited similar viability compared to the control group (54.1±12.6%). In experiment 3, the effects of different lecithin concentrations on viability, acrosomal membrane integrity, and mitochondrial membrane integrity of spermatozoa were examined.
The percentage of live spermatozoa with an intact acrosome and high mitochondrial membrane potential in the 0.1% lecithin group was not significantly different compared to the control group (31.2±13.3 vs. 30.5±10.9%). In experiment 4, the effect of different lecithin concentrations on the plasma membrane integrity of spermatozoa was examined. The percentage of spermatozoa with a normal plasma membrane was similar between the 0.1% lecithin and control groups (31.2±13.3 vs. 30.5±10.9%). In conclusion, we suggest that semen extender supplemented with 0.1% lecithin can replace 20% egg yolk without reducing spermatozoa quality.
목차
등록된 정보가 없습니다.
참고문헌 (40)
참고문헌 신청
Aires VA / 2003 / In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen / Theriogenology 60:269~279
Akhter S / 2012 / Soya-lecithin in extender improves the freezability and fertility of buffalo (Bubalus bubalis)bull spermatozoa / Reprod. Domest. Anim 47:815~819
Allouche L / 2017 / Bull fertility and its relation with density gradient selected sperm / Int. J. Fertil. Steril 11:55~62
Barth AD / 2004 / Use of internal artificial vaginas for breeding soundness evaluation in range bulls: an alternative for electroejaculation allowing observation of sex drive and mating ability / Anim. Reprod. Sci 84:315~325
Bassiri F / 2012 / Is there an association between HOST grades and sperm quality? / Hum. Reprod 27:2277~2284
Akhter S / 2012 / Soya-lecithin in extender improves the freezability and fertility of buffalo (Bubalus bubalis)bull spermatozoa / Reprod. Domest. Anim 47:815~819
Allouche L / 2017 / Bull fertility and its relation with density gradient selected sperm / Int. J. Fertil. Steril 11:55~62
Barth AD / 2004 / Use of internal artificial vaginas for breeding soundness evaluation in range bulls: an alternative for electroejaculation allowing observation of sex drive and mating ability / Anim. Reprod. Sci 84:315~325
Bassiri F / 2012 / Is there an association between HOST grades and sperm quality? / Hum. Reprod 27:2277~2284
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