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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 135 - 140 (6page)
- DOI
- 10.5145/ACM.2021.24.4.4
이용수
초록· 키워드
오류제보하기
SARS-CoV-2의 유전체 분석은 분자역학을 이해하거나 지역사회의 바이러스 전염을 이해하는데 필요하다. 유전자 염기서열분석 기기인 MinION (Oxford Nanopore Technologies, ONT, UK)를 이용하면 다양한 환경에서도 검사를 할 수 있으며, 시설과 자원이 제한된 임상미생물검사실에서도시행할 수 있다. 이 연구에서는 국내의 한 환자에서 검출된 SARS-CoV-2의 유전체 분석을 MinION유전자염기서열 분석기로 분석하였다. 2020년 6월 서울의 한 대학병원에서 SARS-CoV-2 양성을 보인 비인두도말 검체 하나로 MinION 기기와 ARTIC nCoV-2019 sequencing protocol v2를 이용하여 유전체 염기서열 분석을 시행하였다. ONT MinKNOW, RAMPART, Genome Workbench 등의소프트웨어를 사용하여 변이형을 확인하였다. SARS-CoV-2 표준염기서열인 Wuhan-Hu-1 isolate(NC_045512.2)에 대해 분석한 결과 11개의 유전자 변이가 관찰되었으며, ORF1ab 부위에서 6개(T265I, F924, Y3884L, P4715L, L5462, Q6804L), S 유전자에서 1개(D614G), ORF3a 부위에서 1개(Q57H), N 유전자에서 1개(P302), 5′-UTR 와 3′-UTR에서 각각 1개씩 관찰되었다. 유전자 증폭을 통한 유전체 분석이 가능한 MinION 기기는 바이러스 유행시 배양시설이 없는 곳에서 유용한 기법으로서 유전체 분석에 사용할 수 있을 것으로 보인다.
목차
등록된 정보가 없습니다.
참고문헌 (11)
참고문헌 신청
Uhteg K / 2020 / Coronavirus detection in the clinical microbiology laboratory : are we ready for identifying and diagnosing a novel virus / Clin Lab Med 40:459~472
Burki T / 2021 / Understanding variants of SARS-CoV-2 / Lancet 397:462
Kim HM / 2021 / Comparative analysis of 7short-read sequencing platforms using the Korean Reference Genome : MGI and Illumina sequencing benchmark for whole-genome sequencing / Gigascience 10:giab014
Kim JM / 2021 / Genomic investigation of the coronavirus disease-2019 outbreak in the Republic of Korea / Sci Rep 11:1~10
Hourdel V / 2020 / Rapid genomic characterization of SARS-CoV-2 by direct amplicon-based sequencing through comparison of MinION and Illumina iSeq100(TM)system / Front Microbiol 11:571328
Burki T / 2021 / Understanding variants of SARS-CoV-2 / Lancet 397:462
Kim HM / 2021 / Comparative analysis of 7short-read sequencing platforms using the Korean Reference Genome : MGI and Illumina sequencing benchmark for whole-genome sequencing / Gigascience 10:giab014
Kim JM / 2021 / Genomic investigation of the coronavirus disease-2019 outbreak in the Republic of Korea / Sci Rep 11:1~10
Hourdel V / 2020 / Rapid genomic characterization of SARS-CoV-2 by direct amplicon-based sequencing through comparison of MinION and Illumina iSeq100(TM)system / Front Microbiol 11:571328
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