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논문 기본 정보

자료유형
학술저널
저자정보
Sarath Chandra Veerla (Chungnam National University) Da Reum Kim (Chungnam National University) 양성윤 (충남대학교)
저널정보
한국생체재료학회 생체재료학회지 생체재료학회지 제22권 제1호
발행연도
2018.3
수록면
53 - 63 (11page)
DOI
10.1186/s40824-018-0119-9

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초록· 키워드

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Background: Controlled drug delivery system is highly important for not only prolonged the efficacy of drug but also cellular development for tissue engineering. A number of biopolymer composites and nanostructured carriers behave been used for the controlled drug delivery of therapeutics. Recently, in vitro microfluidic devices that mimic the human body have been developed for drug-delivery applications. Methods: A microfluidic channel was fabricated via a two-step process: (i) polydimethyl siloxane (PDMS) and curing agent were poured with a 10:2 mass ratio onto an acrylic mold with two steel pipes, and (ii) calcium alginate beads were synthesized using sodium alginate and calcium chloride solutions. Different amounts (10, 25, 50 μg) of graphene oxide (GO) were then added by Hummers method, and studies on the encapsulation and release of the model drug, risedronate (Ris), were performed using control hydrogel beads (pH 6.3), GO-containing beads (10GO, 25GO and 50GO), and different pH conditions. MC3T3 osteoblastic cells were cultured in a microchannel with Ris-loaded GO-hydrogel beads, and their proliferation, viability, attachment and spreading were assessed for a week. Results: The spongy and textured morphology of pristine hydrogel beads was converted to flowery and rodshaped structures in drug-loaded hydrogel beads at reduced pH (6.3) and at a lower concentration (10 μg) of GO. These latter 10GO drug-loaded beads rapidly released their cargo owing to the calcium phosphate deposited on the surface. Notably, beads containing a higher amount of GO (50GO) exhibited an extended drug-release profile. We further found that MC3T3 cells proliferated continuously in vitro in the microfluidic channel containing the GO-hydrogel system. MTT and live/dead assays showed similar proliferative potential of MC3T3 cells. Therefore, a microfluidic device with microchannels containing hydrogel beads formulated with different amounts of GO and tested under various pH conditions could be a promising system for controlled drug release. Conclusions: The GO and drug (risedronate, Rig) were directed loaded into a hydrogel placed in a microchannel. Through interactions such as hydrogen bonding between Go and the Rig-loaded GO-hydrogel beads, the beadloaded microfluidic device supported MC3T3 proliferation and development as osteoblast without additional osteogenic differentiation supplements.
#Microfluidics #Drug delivery #Hydrogel #Encapsulation #Graphene oxide #Risedronate #Osteoblast cell cultivation

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참고문헌 (28)

참고문헌 신청
Nguyen NT / 2013 / Design, fabrication and characterization of drug delivery systems based on lab-on-a-chip technology / Adv Drug Deliv Rev 65:1403~1419 Crossref Bhatia SN / 2014 / Microfluidic organs-on-chips / Nat Biotechnol 32:760~7752 Crossref Zhou T / 2014 / Controlled release of doxorubicin from graphene oxide based charge-reversal nanocarrier / Biomaterials 35:4185~4194 Crossref Fan L / 2016 / Sodium alginate conjugated graphene oxide as a new carrier for drug delivery system / Int J Biol Macromol 93:582~590 Crossref Wang J / 2014 / Controlled release of anticancer drug using graphene oxide as a drug-binding effector in konjac glucomannan/sodium alginate hydrogels / Colloids Surf B 113:223~229 Crossref

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