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논문 기본 정보

자료유형
학술저널
저자정보
Biswaranjan Behera (ICAR-National Rice Research Institute) Shashikanta Behera (Sambalpur University) Shasmita (Ravenshaw University) Debasish Mohapatra (Ravenshaw University) Durga Prasad Barik (Ravenshaw University) Soumendra Kumar Naik (Ravenshaw University)
저널정보
한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제48권 제4호
발행연도
2021.12
수록면
255 - 263 (9page)
DOI
10.5010/JPB.2021.48.4.255

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Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal commu- nities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl2 (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low tem- perature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 °C; thus, they could be useful for transfer and exchange of P. foetida germplasm.

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