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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 131 - 138 (8page)
- DOI
- 10.5010/JPB.2021.48.3.131
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초록· 키워드
오류제보하기
We reviewed current research trends for discriminating between species of the Angelica genus, a group of important medicinal plants registered in South Korea, China, and Japan. Since the registered species for medicinal purposes differ by country, they are often adulterated as well as mixed in commercial markets. Several DNA technologies have been applied to distinguish between species. However, one of the restrictions is insufficient single-nucleotide polymorphisms (SNPs) within the target DNA fragments; in particular, among closely-related species. Recently, amplification refractory mutation system (ARMS)-PCR and highresolution melting (HRM) curve analysis techniques have been developed to solve such a problem. We applied both technologies, and found they were able to discriminate several lines of Angelica genus, including A. gigas Nakai, A.
gigas Jiri, A. sinensis, A. acutiloba Kitag, and Levisticum officinale. Furthermore, although the ITS region differs only by one SNP between A. gigas Nakai and A. gigas Jiri, both HRM and ARMS-PCR techniques were powerful enough to discriminate between them. Since both A. gigas Nakai and A.
gigas Jiri are native species to South Korea and are very closely related, they are difficult to discriminate by their morphological characteristics. For practical applications of these technologies, further research is necessary with various materials, such as dried or processed materials (jam, jelly, juice, medicinal decoctions, etc.) in commercial markets.
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등록된 정보가 없습니다.
참고문헌 (41)
참고문헌 신청
Blank I / 1993 / Analysis of the seasoning-like flavour substances of a commercial lovage extract (Levisticum officinale Koch.) / Flavour Fragr J 8:191~195
Bylaite E / 1998 / Influence of harvesting time on the composition of volatile components in dierent anatomical parts of lovage (Levisticum officinale Koch.) / J Agric Food Chem 46:3735~3740
Chen C / 2019 / Mining of simple sequence repeats(SSRs)loci and development of novel transferability-across EST-SSR markers from de novo transcriptome assembly of Angelica dahurica / PLoS ONE 14:e0221040
최호영 / 2004 / 當歸類 한약재 감별을 위한 ITS 부위의 염기서열 및 AFLP 분석 / 대한본초학회지 19(2):91~100
Choo BK / 2009 / Development of SCAR markers for the discrimination of three species of medicinal plants, Angelica d ecu rsiva(Peucedanum decursivum), Peucedanum praeruptorum and Anthricus sylvestris, based on the internal transcribed spacer(ITS)sequence and random amplified polymorphic DNA(RAPD) / Biol Pharm Bull 32:24~30
Bylaite E / 1998 / Influence of harvesting time on the composition of volatile components in dierent anatomical parts of lovage (Levisticum officinale Koch.) / J Agric Food Chem 46:3735~3740
Chen C / 2019 / Mining of simple sequence repeats(SSRs)loci and development of novel transferability-across EST-SSR markers from de novo transcriptome assembly of Angelica dahurica / PLoS ONE 14:e0221040
최호영 / 2004 / 當歸類 한약재 감별을 위한 ITS 부위의 염기서열 및 AFLP 분석 / 대한본초학회지 19(2):91~100
Choo BK / 2009 / Development of SCAR markers for the discrimination of three species of medicinal plants, Angelica d ecu rsiva(Peucedanum decursivum), Peucedanum praeruptorum and Anthricus sylvestris, based on the internal transcribed spacer(ITS)sequence and random amplified polymorphic DNA(RAPD) / Biol Pharm Bull 32:24~30
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