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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.11
- 수록면
- 1,032 - 1,041 (10page)
- DOI
- 10.3349/ymj.2021.62.11.1032
이용수
초록· 키워드
오류제보하기
Purpose: The ferric chloride (FeCl3)-induced thrombosis model is widely used for thrombosis research. However, it lacks standardization with uncertainty in the exact mechanism of thrombosis. This study aimed to characterize thrombus formation in a mouse model.
Materials and Methods: We investigated thrombus formation and stability using various FeCl3 concentrations (10%, 20%, 30%, 40%, and 50%, w/v) in carotid arteries of the Institute of Cancer Research (ICR) and C57BL/6N mice using the FeCl3-induced thrombosis model. We also investigated thrombus histopathology using immunohistochemistry and electron microscopy.
Results: Higher FeCl3 concentrations induced dose-dependent, faster, larger, and more stable thrombus formation in both strains of mice. However, the ICR mice showed better dose-responses in thrombus formation and stability compared to the C57BL/6N mice. Thrombi were fibrin- and platelet-rich without significant changes across FeCl3 concentrations. However, the content of red blood cells (RBCs) increased with increasing FeCl3 concentrations (p for trend <0.001) and inversely correlated with time to occlusion (r=-0.65, p<0.001). While platelets and fibrin were evenly distributed over the thrombus, RBCs were predominantly located near the FeCl3 treatment area. Transmission electron microscopy showed that RBCs attached to and were surrounded by aggregates of degranulated platelets, suggesting their potential role in platelet activation.
Conclusion: Faster and larger thrombus formation is induced in a dose-dependent manner by a wide range of FeCl3 concentrations, but the stable thrombus formation requires higher FeCl3 concentrations. Mouse strain affects thrombus formation and stability. RBCs and their interaction with platelets play a key role in the acceleration of FeCl3-induced thrombosis.
목차
등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Bonnard T / 2015 / Ferric chloride-induced thrombosis mouse model on carotid artery and mesentery vessel / J Vis Exp :e52838
Li W / 2013 / Ferric chloride-induced murine carotid arterial injury : a model of redox pathology / Redox Biol 1:50~55
Li W / 2016 / Ferric chloride-induced murine thrombosis models / J Vis Exp :54479
Konstantinides S / 2001 / Plasminogen activator inhibitor-1 and its cofactor vitronectin stabilize arterial thrombi after vascular injury in mice / Circulation 103:576~583
Kwon I / 2013 / Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods : a FeCl3-induced thrombosis model in mice / PLoS One 8:e81165
Li W / 2013 / Ferric chloride-induced murine carotid arterial injury : a model of redox pathology / Redox Biol 1:50~55
Li W / 2016 / Ferric chloride-induced murine thrombosis models / J Vis Exp :54479
Konstantinides S / 2001 / Plasminogen activator inhibitor-1 and its cofactor vitronectin stabilize arterial thrombi after vascular injury in mice / Circulation 103:576~583
Kwon I / 2013 / Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods : a FeCl3-induced thrombosis model in mice / PLoS One 8:e81165
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