지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.11
- 수록면
- 1,423 - 1,432 (10page)
- DOI
- 10.5187/jast.2021.e124
이용수
초록· 키워드
오류제보하기
To elucidate the role and mechanism of microbes, we combined culture-dependent and culture- independent approaches to investigate differences in gut bacterial composition between sows and weaned pigs. Under anaerobic conditions, several nonselective and selective media were used for isolation from fecal samples. All isolated bacteria were identified and classified through 16S rRNA sequencing, and the microbiota composition of the fecal samples was analyzed by metagenomics using next generation sequencing (NGS) technology. A total of 278 and 149 colonies were acquired from the sow and weaned pig fecal samples, respectively. Culturomics analysis revealed that diverse bacterial genus and species belonged to Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes were isolated from sow and weaned pigs. When comparing culture-dependent and culture-independent analyses, 191 bacterial species and 2 archaeal bacterial species were detected through culture-independent analysis, and a total of 23 bacteria were isolated through a culture-dependent approach, of which 65% were not detected by metagenomics. In conclusion, culturomics and metagenomics should be properly combined to fully understand the intestinal microbiota, and livestock-derived microbial resources should be informed by culturomic approaches to understand and utilize the mechanism of host-microbe interactions.
목차
등록된 정보가 없습니다.
참고문헌 (36)
참고문헌 신청
Junjie Qin / 2010 / A human gut microbial gene catalogue established by metagenomic sequencing / Nature 464(7285):59~65
Valentina Tremaroli / 2012 / Functional interactions between the gut microbiota and host metabolism / Nature 489(7415):242~249
Inna Sekirov / 2010 / Gut Microbiota in Health and Disease / Physiological Reviews 90(3):859~904
June L. Round / 2009 / The gut microbiota shapes intestinal immune responses during health and disease / Nature Reviews Immunology 9(5):313~323
Lora V. Hooper / 2012 / Interactions Between the Microbiota and the Immune System / Science 336(6086):1268~1273
Valentina Tremaroli / 2012 / Functional interactions between the gut microbiota and host metabolism / Nature 489(7415):242~249
Inna Sekirov / 2010 / Gut Microbiota in Health and Disease / Physiological Reviews 90(3):859~904
June L. Round / 2009 / The gut microbiota shapes intestinal immune responses during health and disease / Nature Reviews Immunology 9(5):313~323
Lora V. Hooper / 2012 / Interactions Between the Microbiota and the Immune System / Science 336(6086):1268~1273
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