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논문 기본 정보

자료유형
학술저널
저자정보
Liu Wei-Bing (Lab of Biosystems and Microanalysis Biomedical Nanotechnology Center State Key Laboratory of Biorea) Lin Zhi-Wei (Lab of Biosystems and Microanalysis Biomedical Nanotechnology Center State Key Laboratory of Biorea) Zhou Ying (Lab of Biosystems and Microanalysis Biomedical Nanotechnology Center State Key Laboratory of Biorea) Ye Bang-Ce (Lab of Biosystems and Microanalysis Biomedical Nanotechnology Center State Key Laboratory of Biorea)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제11호
발행연도
2021.11
수록면
1,545 - 1,551 (7page)
DOI
10.4014/jmb.2101.01026

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초록· 키워드

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Exopolysaccharides (EPSs) such as capsular polysaccharide (CPS) are important bioactive carbohydrate compounds and are often used as bioenrichment agents and bioabsorbers to remove environmental pollutants like di-n-butyl phthalate (DBP). Among the EPS-producing bacteria, lactic acid bacteria (LAB) have gained the most attention. As generally recognized as safe (GRAS) microorganisms, LAB can produce EPSs having many different structures and no health risks. However, EPS production by LAB does not meet the needs of large-scale application on an industrial scale. Here, the capA gene (encoding CPS biosynthesis protein) was overexpressed in Lactobacillus plantarum P1 to improve the production of EPSs and further enhance the DBP adsorption capability. Compared with P1, the CPS production in capA overexpressed strain was increased by 11.3 mg/l, and the EPS thickness was increased from 0.0786 ± 0.0224 μm in P1 to 0.1160 ± 0.0480 μm in P1-capA. These increases caused the DBP adsorption ratio of P1-capA to be doubled. Overall, the findings in this study provide a safe method for the adsorption and removal of DBP.

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