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논문 기본 정보

자료유형
학술저널
저자정보
Wang Jing (TEDA Institute of Biological Sciences and Biotechnology Nankai University 23 Hongda Street TEDA Tia) Jiao HongBo (LanLing Center for Disease Control and Prevention 1 City Huibao Road Lanling 276000 Lanling Shandon) Zhang XinFeng (Taian Center for Disease Control and Prevention 33 Changcheng Road Taian 271000 Shandong P.R China) Zhang YuanQing (Jinan KeJia Medical Laboratory Inc. 800 Minghu West Road Jinan 250001 Shandong P.R China) Sun Na (Shandong Center for Disease Control and Prevention 16992 City Ten Road Jinan 250014 Shandong P.R Ch) Ying Yang (Shandong Center for Disease Control and Prevention 16992 City Ten Road Jinan 250014 Shandong P.R Ch) Wei Yi (TEDA Institute of Biological Sciences and Biotechnology Nankai University 23 Hongda Street TEDA Tia) Hu Bin (Shandong Center for Disease Control and Prevention 16992 City Ten Road Jinan 250014 Shandong P.R Ch) Guo Xi (TEDA Institute of Biological Sciences and Biotechnology Nankai University 23 Hongda Street TEDA Tia)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제9호
발행연도
2021.9
수록면
1,191 - 1,199 (9page)
DOI
10.4014/jmb.2105.05016

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Enteropathogenic Escherichia coli (EPEC), which belongs to the attaching and effacing diarrheagenic E. coli strains, is a major causative agent of life-threatening diarrhea in infants in developing countries. Most EPEC isolates correspond to certain O serotypes; however, many strains are nontypeable. Two EPEC strains, EPEC001 and EPEC080, which could not be serotyped during routine detection, were isolated. In this study, we conducted an in-depth characterization of their putative O-antigen gene clusters (O-AGCs) and also performed constructed mutagenesis of the O-AGCs for functional analysis of O-antigen (OAg) synthesis. Sequence analysis revealed that the occurrence of O-AGCs in EPEC001 and E. coli O132 may be mediated by recombination between them, and EPEC080 and E. coli O2/O50 might acquire each O-AGC from uncommon ancestors. We also indicated that OAgknockout bacteria were highly adhesive in vitro, except for the EPEC001 wzy derivative, whose adherent capability was less than that of its wild-type strain, providing direct evidence that OAg plays a key role in EPEC pathogenesis. Together, we identified two EPEC O serotypes in silico and experimentally, and we also studied the adherent capabilities of their OAgs, which highlighted the fundamental and pathogenic role of OAg in EPEC.

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