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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.3
- 수록면
- 1 - 9 (9page)
- DOI
- 10.17642/jcc.27.1.1
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초록· 키워드
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This study was conducted to investigate the protease expression patterns of the novel chitinase producing bacterium Pedobacter sp. PR-M6. Protease were detected on SDS-PAGE gel as condition of 4o C, 25oC and 37oC to activate of enzymes obtained from incubation of LB, NB and 1% skim milk medium at 4oC, 20oC and 30o C after 21 days of incubation, respectively. Pedobacter sp. PR-M6 displayed proteolytic activity on SDS-PAGE gels containing 0.1% (w/v) gelatin, and protease isozymes of PR-M6 were expressed as two major bands (P7 and P8) and seven minor bands (P1, P2, P3, P4, P5, P6, and P9). Protease isozymes of PR-M6 were inhibited at 4o C and 25o C. The two protease isozymes P5 and P6 were newly expressed in the LB culture medium at 20o C. To investigate the properties of a heat-stable antifungal factor (HSAF), PR-M6 stain was cultured to obtain the products of biosynthesis for incubation in LB medium. HSAF exhibited active biosynthesis at 20o C for incubation in LB medium. Crude HASF obtained from LB20 medium showed hyphal growth inhibition of Rhizoctonia solani.
Inhibition rate of R. solani growth was as high as 67% at 24 h and 47.8% at 48 h after incubation with 1,000 ppm of crude HSAF. Trypan blue stained the inside of the edge for destructed R. solani cell walls after crude HSAF treatment. These results indicated that HSAF and protease from Pedobacter sp. PR-M6 could be used as biocontrol agents against phytopathogens in agriculture
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