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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.8
- 수록면
- 913 - 922 (10page)
- DOI
- 10.1007/s13258-021-01208-7
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초록· 키워드
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Background Disruption of ICT1 has been known to cause a significant deficiency in the phospholipid composition which is necessary for cell stress adaptation. However, the effects of ICT1 deletion on antioxidant research are not clear.
Objective Construct a knockout strain to investigate the efficacy of ICT1 on antioxidant activity.
Methods The antioxidant-related genes and phospholipid-related genes were determined by RT-PCR, the cell wall shape was observed by TME, and CWI pathway phosphorylation experiments were also analyzed by HPLC.
Results The expression of antioxidant related genes and phospholipid-related genes has a slight reduction compared to the wild type. The cell wall was observed impaired with apparent CWI pathway phosphorylation weakening in the mutant.
Conclusion These findings indicate the role of ICT1 on antioxidant activity because it not only directly affects phospholipid composition but also further leads to the activation of CWI.
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