지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제9호
- 발행연도
- 2022.9
- 수록면
- 1,209 - 1,216 (8page)
- DOI
- 10.4014/jmb.2207.07058
이용수
초록· 키워드
오류제보하기
To better understand the effects of PEGylation and biotinylation on the delivery efficiency of proteins, the cationic protein lysozyme (LZ) and anionic protein bovine serum albumin (BSA) were chemically conjugated with poly(ethylene glycol) (PEG) and biotin-PEG to primary amine groups of proteins using N-hydroxysuccinimide reactions. Four types of protein conjugates were successfully prepared: PEGylated LZ (PEG-LZ), PEGylated BSA (PEG-BSA), biotin-PEG-conjugated LZ (Bio-PEGLZ), and biotin-PEG-conjugated BSA (Bio-PEG-BSA). PEG-LZ and Bio-PEG-LZ exhibited a lower intracellular uptake than that of LZ in A549 human lung cancer cells (in a two-dimensional culture).
However, Bio-PEG-BSA showed significantly improved intracellular delivery as compared to that of PEG-BSA and BSA, probably because of favorable interactions with cells via biotin receptors. For A549/fibroblast coculture spheroids, PEG-LZ and PEG-BSA exhibited significantly decreased tissue penetration as compared with that of unmodified proteins. However, Bio-PEG-BSA showed tissue penetration comparable to that of unmodified BSA. In addition, citraconlyated LZ (Cit-LZ) showed reduced spheroid penetration as compared to that of LZ, probably owing to a decrease in protein charge. Taken together, chemical conjugation of targeting ligands-PEG to anionic proteins could be a promising strategy to improve intracellular delivery and in vivo activity, whereas modifications of cationic proteins should be more delicately designed.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Amine functionalized plasma polymerized PEG film : Elimination of non-specific binding for biosensing
한국진공학회 학술발표회초록집
2016 .02
-
DSSCs efficiencies of PEG in TiO₂ paste
한국진공학회 학술발표회초록집
2016 .08
-
건조온도와 아세틸화처리가 소나무 원판의 Polyethylene Glycol 잔류량에 미치는 효과
목재공학(Journal of the Korean Wood Science and Technology)
2015 .01
-
Fabrication and Characterization of Plasma polymerized PEG on Stainless Beads by using Plasma-enhanced Chemical Vapor Depostion
한국진공학회 학술발표회초록집
2017 .02
-
Fabrication and characterization of carboxyl-functionalized PEG thin films by plasma polymerization
한국진공학회 학술발표회초록집
2016 .08
-
공생별 AG Peg의 H 방출선 윤곽 분석
한국지구과학회지
2017 .01
-
High-Temperature Drying of Bamboo Tubes Pretreated with Polyethylen Glycol Solution
목재공학(Journal of the Korean Wood Science and Technology)
2017 .01
-
A novel nonsense mutation in NPR2 gene causing Acromesomelic dysplasia, type Maroteaux in a consanguineous family in Southern Punjab (Pakistan)
Genes & Genomics
2020 .01
-
AG Peg의 분광 관측과 Bowen 형광 기작의 효율 변화
한국지구과학회지
2017 .01
-
PEGylated graphene oxide-based colorimetric sensor for recording temperature
한국진공학회 학술발표회초록집
2021 .02
-
추출조건에 따른 아마란스 잎(Amaranthus cruentus L.) 첨가 소시지의 단백질 함량비교
동아시아식생활학회 학술발표대회논문집
2016 .10
-
Novel Approach of a Phage-Based Magnetoelastic Biosensor for the Detection of Salmonella enterica serovar Typhimurium in Soil
Journal of Microbiology and Biotechnology
2016 .12
-
Study of cellular behaviors on hydrophobic PDMS (poly dimethylsiloxane) nano-brush by tracking FA complexes using BSA-coated ZAIS nanoparticle probe
한국분석과학회 학술대회
2017 .11
-
Purification, crystallization, and X-ray crystallographic analysis of Vac8p complexed with Atg13p from Saccharomyces cerevisiae
Biodesign
2017 .09
-
표면처리에 의한 폴리에틸렌 글리콜(PEG) Õ 덱스트란(DEX) 수용성 액체 이상 시스템에서의 마이크로 입자의 분배 조절
한국분석과학회 학술대회
2015 .11
-
Soluble Expression of Artificially Designed Recombinant Protein through Conjugating Fusion Protein in Escherichia coli
한국미생물학회 학술대회논문집
2017 .04
-
Regional differences in protein intake and protein sources of Korean older adults and their association with metabolic syndrome using the 2016–2019 Korea National Health and Nutrition Examination Surveys: a cross-sectional study
대한지역사회영양학회지
2024 .06
-
Two-dimensional correlation analysis in polymer and protein studies
한국분석과학회 학술대회
2016 .11
-
A review of recent evidence of dietary protein intake and health
Nutrition Research and Practice
2022 .05
-
A review of recent evidence of dietary protein intake and health
Nutrition Research and Practice
2022 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0