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논문 기본 정보

자료유형
학술저널
저자정보
Li Ying-Bin (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultura) Zhang Zhi-Ping (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultura) Yuan Ye (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultura) Huang Hui-Chuan (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultura) Mei Xin-Yue (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultura) Du Fen (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultura) Yang Min (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultura) Liu Yi-Xiang (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultura) Zhu Shu-Sheng (State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan Agricultura)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제3호
발행연도
2022.3
수록면
294 - 301 (8page)
DOI
10.4014/jmb.2112.12005

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In our greenhouse experiment, soil heat treatment groups (50, 80, and 121°C) significantly promoted growth and disease suppression of Panax notoginseng in consecutively cultivated soil (CCS) samples (p < 0.01), and 80°C worked better than 50°C and 121°C (p < 0.01). Furthermore, we found that heat treatment at 80°C changes the microbial diversity in CCS, and the inhibition ratios of culturable microorganisms, such as fungi and actinomycetes, were nearly 100%. However, the heat-tolerant bacterial community was preserved. The 16S rRNA gene and internal transcribed spacer (ITS) sequencing analyses indicated that the soil heat treatment had a greater effect on the Chao1 index and Shannon’s diversity index of bacteria than fungi, and the relative abundances of Firmicutes and Proteobacteria were significantly higher than without heating (80 and 121°C, p < 0.05). Soil probiotic bacteria, such as Bacillus (67%), Sporosarcina (9%), Paenibacillus (6%), Paenisporosarcina (6%), and Cohnella (4%), remained in the soil after the 80°C and 121°C heat treatments. Although steam increased the relative abundances of most of the heat-tolerant microbes before sowing, richness and diversity gradually recovered to the level of CCS, regardless of fungi or bacteria, after replanting. Thus, we added heat-tolerant microbes (such as Bacillus) after steaming, which reduced the relative abundance of pathogens, recruited antagonistic bacteria, and provided a long-term protective effect compared to the steaming and Bacillus alone (p < 0.05). Taken together, the current study provides novel insight into sustainable agriculture in a consecutively cultivated system.

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