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논문 기본 정보

자료유형
학술저널
저자정보
Migyung Cho (Tongmyong University)
저널정보
한국정보통신학회JICCE Journal of information and communication convergence engineering Journal of information and communication convergence engineering Vol.13 No.3
발행연도
2015.9
수록면
215 - 219 (5page)

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Cells in a culture process transform with time and produce many overlapping cells in their vicinity. We are interested in a separation algorithm for images of overlapping cells taken using a fluorescence optical microscope system during a cell culture process. In this study, all cells are assumed to have an ellipse-like shape. For an ellipse fitting-based method, an improved least squares method is used by decomposing the design matrix into quadratic and linear parts for the separation of overlapping cells. Through various experiments, the improved least squares method (numerically stable direct least squares fitting [NSDLSF]) is compared with the conventional least squares method (direct least squares fitting [DLSF]). The results reveal that NSDLSF has a successful separation ratio with an average accuracy of 95% for two overlapping cells, an average accuracy of 91% for three overlapping cells, and about 82% accuracy for four overlapping cells.

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Abstract
I. INTRODUCTION
II. MIXED THRESHOLD METHOD
III. SEPARATION OF OVERLAPPING CELLS BASED ON ELLIPSE FITTING
IV. EXPERIMENTAL RESULTS
V. CONCLUSION
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