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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.10
- 수록면
- 21 - 29 (9page)
- DOI
- 10.5207/JIEIE.2022.36.10.021
이용수
초록· 키워드
오류제보하기
In this paper, we demonstrated the detection of SARS-CoV-2 spike protein using a fiber-optic biosensor composed of tapered no-core fiber (NCF), which leads to multi-mode interference (MMI) when its both ends are spliced with standard single-mode fiber (SMF). For the fabrication of the tapered NCF as a bare sensor head, we utilized a commercial arc fusion splicer (AFS) and optimized the tapering condition by adjusting a pull speed, an arc duration, and a pull length of the AFS, resulting in a tapered core diameter of ∼105μm. To check if the bare sensor head is feasible for the SARS-CoV spike protein detection, the response of the tapered NCF (whose both ends are connected with SMF) to the external refractive index (RI) was investigated using an sucrose solution. It was confirmed from the RI examination that two loss dips selected from the MMI spectrum of the bare sensor head, designated as Dip A and Dip B, showed the RI sensitivities of ∼65.60 and ∼73.01nm/RIU, respectively. Then, we immobilized human ACE2 protein on the fiber surface of the bare sensor head to increase the binding efficiency of the sensor head with SARS-CoV-2 spike protein. Finally, we experimentally demonstrated that the proposed sensor could detect SARS-CoV-2 spike protein with the sensitivities of 54.42 and 79.55pm/(μg/ml) for Dip A and Dip B, respectively.
목차
Abstract
1. 서론
2. ACE 2 항체 고정용 화학 약품
3. 센서 제작 및 측정 원리
4. 표면 항체 고정 과정 및 SARS-CoV-2 스파이크 단백질 검출 결과
5. 결론
References
참고문헌 (18)
참고문헌 신청
D. Kim, et al., “The Architecture of SARS-CoV-2 Transcriptome,” Cell, Vol. 181, No. 4, pp. 914-921, 2020.
G. Emmi, et al., “SARS-CoV-2 Infection Among Patients with Systemic Autoimmune Diseases,” Autoimmun. Rev., Vol. 19, No. 7, p. 102575, 2020.
M. Janczuk-Richter, et al., “Immunosensor Based on Long-Period Fiber Gratings for Detection of Viruses Causing Gastroenteritis,” Sensors, Vol. 20, No. 3, pp. 1-11, 2020.
M. Janczuk-Richter, et al., “Long-period Fiber Grating Sensor for Detection of Viruses,” Sens. Actuators B Chem., Vol. 250, pp. 32-38, 2017.
L. L. Liu, et al., “Highly Sensitive Label-free Antibody Detection Using a Long Period Fibre Grating Sensor,” Sens. Actuators B Chem., Vol. 271, pp. 24-32, 2018.
G. Emmi, et al., “SARS-CoV-2 Infection Among Patients with Systemic Autoimmune Diseases,” Autoimmun. Rev., Vol. 19, No. 7, p. 102575, 2020.
M. Janczuk-Richter, et al., “Immunosensor Based on Long-Period Fiber Gratings for Detection of Viruses Causing Gastroenteritis,” Sensors, Vol. 20, No. 3, pp. 1-11, 2020.
M. Janczuk-Richter, et al., “Long-period Fiber Grating Sensor for Detection of Viruses,” Sens. Actuators B Chem., Vol. 250, pp. 32-38, 2017.
L. L. Liu, et al., “Highly Sensitive Label-free Antibody Detection Using a Long Period Fibre Grating Sensor,” Sens. Actuators B Chem., Vol. 271, pp. 24-32, 2018.
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