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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.9
- 수록면
- 192 - 195 (4page)
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초록· 키워드
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An antibacterial benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, isolated from Ficus foveolata stems was used as a standard marker for establishment of quantitative HPLC analysis for the stem extracts of F. foveolata . The method utilized a TSK-gel ODS-80Ts column (5 μm, 4.6 × 250 mm) with the mixture of methanol and 5% acetic acid in water (24:76, v/v) as the mobile phase at a flow rate of 1 mL/min, and quantitative detection at 289 nm. The parameters i.e. linearity, intraday and interday precision, accuracy, specificity and sensitivity of the method were evaluated for method validation. The recoveries of the method were 99.5 - 103.6% and good linearity (R<sup>2</sup> ≥ 0.9999) was obtained. A high degree of specificity, sensitivity as well as repeatability and reproducibility (RSD less than 2 and 5%, respectively) were also achieved. Chloroform was served as the most suitable solvent for extraction of 2,6-dimethoxy-1,4-benzoquinone. The optimised sample preparation and HPLC method can be practically used in the routine quality control process of F. foveolata stem extracts.
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참고문헌 (7)
참고문헌 신청
Chaudhary, L. B / 2012 / S. Taiwania 57:193~216
Suksri, S / 2005 / Kasetsart J 39:519~533
Meerungrueang, W / 2014 / Pharm. Biol 52:1104~1109
김명희 / 2010 / Antimicrobial Activities of 1,4-Benzoquinones and Wheat Germ Extract / Journal of Microbiology and Biotechnology 20(8):1204~1209
Pethig, R / 1983 / Proc. Natl. Acad. Sci. U.S.A 80:129~132
Suksri, S / 2005 / Kasetsart J 39:519~533
Meerungrueang, W / 2014 / Pharm. Biol 52:1104~1109
김명희 / 2010 / Antimicrobial Activities of 1,4-Benzoquinones and Wheat Germ Extract / Journal of Microbiology and Biotechnology 20(8):1204~1209
Pethig, R / 1983 / Proc. Natl. Acad. Sci. U.S.A 80:129~132
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