지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국농약과학회 농약과학회지 농약과학회지 제26권 제1호
- 발행연도
- 2022.3
- 수록면
- 74 - 82 (9page)
- DOI
- 10.7585/kjps.2022.26.1.74
이용수
초록· 키워드
오류제보하기
Tobacco cutworm (Spodoptera litura [Fabricius]) is a destructive insect pest distributed in densely cultivated parts of Asia and Australia. This insect shows an explosive increase in its population in summer. Furthermore, tobacco cutworm larvae are very difficult to control because of their strong resistance to commonly applied chemical insecticides. To identify alternative pesticides that circumvent toxic effects to the environment and the threat of insecticide resistance, we aimed to examine plant extracts with insect growth regulatory activity targeting juvenile hormones (JHs). Using RNA sequencing and de novo transcriptome assembly, S. litura cDNA sequences of the JH receptor methoprene-tolerant (Met) and steroid receptor coactivator (SRC) were determined and used to generate yeast two-hybrid bait and prey plasmids. The JH/JH analog-mediated binding of Met and SRC was measured by assaying β-galactosidase activity. In the plant extract activity assay, we tested 1,628 plant extracts in transformants bearing Met and SRC. We identified 67 plant extracts with high JH disruptor (JHD) activity in tobacco cutworm, of which Gladiolus gandavensis extract showed the highest JHD activity. Plant-based JHDs might serve as a viable and sustainable alternative to chemical pesticides to control tobacco cutworm infestations in the field.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Literature cited
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