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논문 기본 정보

자료유형
학술저널
저자정보
Yong Hwan Jung (Cheju National University) Moon You Oh (Cheju National University)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.27 No.4
발행연도
2005.1
수록면
279 - 288 (10page)

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초록· 키워드

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We used sequences from both rbcL gene and trnL-trnF intergenic spacer of chloroplast DNA (cpDNA) for phylogenetic reconstruction of 39 acesions of PrunusJapan. No sequence length variation was found in the 39 acesions based on rbcL gene sequences. However, the length of the trnL-trnF spacer ranged from 358 to 387 base pairs (bp). Of the 1,790 aligned positions of the two cpDNA regions, 406 sites (22.7%) were rbcL and 107 sites (27.3%, 107/392) in the trnL-trnF intergenic spacer. The longest length mutation of the trnL-trnF spacer was a 21-bp deletion in P. padus and two taxa of P. buergeriana. Phylogenetic trees based on the sequence data were trap values for individual clades based on 1,000 replicates ranged from 80 to 100%. The strict consensus tree had five large, independent groups. The trees contained monophyletic groups consistent with the subgenera, Cerasus, Padus, Amygdalus, Prunophora, and Laurocerasusancestral divergence point with a bootstrap value of 100%. In adition, subgenera Amygdalus and Prunophora had a well-suported sister group relationship with 10% bots trap suport, a s d i d t h e c l a d e o f s u b g e n e r a Cerasus, Laurocerasus a n d Padus. The two natives of P. from Jeju, Korea were clearly distinguished from the cultivars as sugested in previous reports. Accordingly, cpDNA sequence analysis is a useful approach for reconstructing phylogenetic relationships among members of the genus Prunus distributed in Korea and Japan.

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