지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이 논문의 연구 히스토리 (2)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
In this study the polymerase chain reaction (PCR) combined with denaturing gradient gel electrophoresis (DGGE) was evaluated as a method permitting the rapid detection of pathogens in fresh originally grown vegetables. A universal primer (341GCf/534r) was selected for its ability to amplify the V3 region of 16S-rRNA genes in their target pathogens (Salmonella typhimurium, Pseudomonas fluorescens, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, E. coli). The 194 bp fragments in PCR were successfully duplicated as expected. The amplified fragments of the same size from six different pathogens also showed good separation upon DGGE. The detection limit of PCR-DGGE for six pathogens in fresh-cut lettuces were over 10^5 CFU/g when sampled by stomaching. However, when the sampling method was changed from stomaching to shaking, the detection limit of six pathogens in organic vegetables was shown to increase by over 10^1 CFU/g, but only those of B. cereus were over 10^3 CFU/g. Therefore, PCR-DGGE was shown to be a reliable method for the detection of pathogens in fresh-cut vegetables.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Ampe F / 2001 / Dynamics of the microbial community responsible for traditional sour cassava starch fermentation studied by denaturing gradient gel electrophoresis and quantitative rRNA hybridization / Int. J. Food Microbial 65:45~54
Bennett A.R / 1998 / Rapid and definitive detection of Salmonella in food by PCR / Lett. Appl. Microbial 26:437~441
Booysen C / 2002 / Isolation, identification and changes in the composition of lactic acid bacteria during the malting of two different barely cultivars / Int. J. Food Microbial 76:63~73
Cocolin L / 2001 / Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages / Appl. Environ. Microbiol 67:5113~5121
Como-Sabetti K / 1997 / Outbreaks of E.coli O157:H7 associated with eating alfalfa sprouts- Michigan and Virginia / Morbidity and Mortality Weekly Report 46:741~744
Bennett A.R / 1998 / Rapid and definitive detection of Salmonella in food by PCR / Lett. Appl. Microbial 26:437~441
Booysen C / 2002 / Isolation, identification and changes in the composition of lactic acid bacteria during the malting of two different barely cultivars / Int. J. Food Microbial 76:63~73
Cocolin L / 2001 / Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages / Appl. Environ. Microbiol 67:5113~5121
Como-Sabetti K / 1997 / Outbreaks of E.coli O157:H7 associated with eating alfalfa sprouts- Michigan and Virginia / Morbidity and Mortality Weekly Report 46:741~744
이 논문의 저자 정보
최근 본 자료
댓글(0)
0