지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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A multiplex polymerase chain reaction method was developed for rapid detection and identification of Shiga-like toxin producing Escherichia coli O157:H7 in food. Specificity of 15 primer sets that target genes specific for E. coli O157:H7 were tested on five E. coli O157:H7 strains, three other serotypes of E. coli and ten non-E. coli strains using singleplex PCR. Among them, four primer sets for the E. coli O157:H7 genes rfbE, eaeA, stxI and stxII genes were selected for a multiplex PCR assay. All E. coli O157:H7 examined were positive for the rfbE and eaeA genes, however, the amplifications of Shiga-like toxin genes stxI and stxII were dependent on the strains. The detection limit of the multiplex PCR assay was 2.2x101CFU/reaction in artificially inoculated lettuce without enrichment. This multiplex PCR method could be used to check the presence of E. coli O157:H7 and Shiga-like toxin producing E. coli strains in food.
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참고문헌 (20)
참고문헌 신청
Ajmi DA / 2005 / Evaluation of a PCR detection method for Escherichia coli O157:H7/H- bovine faecal samples / Appl. Microbiol 42(4):386~391
Beuchat LR / 2002 / Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables / Microbes Infect 4(4):413~423
Bertrand, R. / 2006 / Evaluation of enrichment-free PCR-based detection on the rfbE gene of Escherichia coli O157-Application to municipal waste water / Water res 41(6):1280~1286
Cebula TA / 1995 / Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their shiga-like toxin type by mismatch amplification mutation assaymultiplex PCR / J. Clin. Microbiol 33(1):248~250
Deisingh AK / 2004 / Strategies for the detection of Escherichia coli O157:H7 in foods / J Appl Microbiol 96(3):419~429
Beuchat LR / 2002 / Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables / Microbes Infect 4(4):413~423
Bertrand, R. / 2006 / Evaluation of enrichment-free PCR-based detection on the rfbE gene of Escherichia coli O157-Application to municipal waste water / Water res 41(6):1280~1286
Cebula TA / 1995 / Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their shiga-like toxin type by mismatch amplification mutation assaymultiplex PCR / J. Clin. Microbiol 33(1):248~250
Deisingh AK / 2004 / Strategies for the detection of Escherichia coli O157:H7 in foods / J Appl Microbiol 96(3):419~429
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