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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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The key enzyme of methanotrophs, methane monooxygenase(MMO) , especially the soluble MMO(sMMO), is remarkable in its broad substrate specificity. Soluble methane monooxygenasee(sMMO) can degrade many chlorinated and aromatic polluants. For this reason, methanotrophic bioreactors with high sMMO activities should be developed to treat these pollutants. Therefore, In this study, production characteristics of soluble methane monooxygenase during growth of methanotrophs in Methylomonas was Investigated. sMMO in methanotrophs of Methylomonas genus is present depending on the copper concentration in the medium, produced only in medium without copper ion. The optimum condition for the production of sMMO was 20:10 methane/Oxygen percent volume ratio. Ammonium salt clearly inhibited the activity of sMMO, but addition of formate the ammonia inhibition action considerable decreased. Also, It was concluded that methanol can be employed to produce methanotrophs in Methylomonas genus containing sMMO.
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참고문헌 (21)
참고문헌 신청
Dalton, H / 1992 / Methane oxidation by methanotrophs: physiological and mechanistic implications / Methane and methanol utilizers. Plenum Press :85~114
Dalton, H / 1985 / Methane oxidation by microorganisms / Microbial gas metabolism. Academic Press Ltd., :173~200
Dalton, H / 1991 / Structure and mechanism of action of the enzymes involved in methane oxidation / Applications of enzyme biotechnology. Plenum Press :55~68
Prior, S. D / 1985 / "The effect of copper ions on membrane content and methane monooxygenase activity in methanol-grown cells of Methylococcus capsulatus (Bath)" / J. Gen. Microbiol 131:155~163
Dalton, H. / 1992 / Methane oxidation by methanotrophs: physiological and mechanistic implications / Methane and methanol utilizers. Plenum Press :85~114
Dalton, H / 1985 / Methane oxidation by microorganisms / Microbial gas metabolism. Academic Press Ltd., :173~200
Dalton, H / 1991 / Structure and mechanism of action of the enzymes involved in methane oxidation / Applications of enzyme biotechnology. Plenum Press :55~68
Prior, S. D / 1985 / "The effect of copper ions on membrane content and methane monooxygenase activity in methanol-grown cells of Methylococcus capsulatus (Bath)" / J. Gen. Microbiol 131:155~163
Dalton, H. / 1992 / Methane oxidation by methanotrophs: physiological and mechanistic implications / Methane and methanol utilizers. Plenum Press :85~114
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