지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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In order to investigate the effect of maturity of seed and embryos, optimal explants and plant growth regulators on embryogenic callus induction and somatic embryogenesis, embryos of Camellia sinensis L. sampled from August to October, 2005 were cultured on MS medium containing various plant growth regulators. Embryogenic cultures were initiated from Camellia sinensis L. immature zygotic embryos. Using a variety of different explants,including whole embryo axis, epicotyl and hypocotyl from immature seed were isolated and cultured on modified MS medium with various combination of plant growth regulators. Developmental stage of zygotic embryos had a large influence on embryogenic callus induction and embryogenesis. Seeds sampled at October, whitish yellow or slight yellow color of embryos(10-15mm size) optimized for tissue culture. Cotyledonary embryo stage optimized for embryogenic callus induction and somatic embryosgenesis. The greatest frequency(85-89%) of embryogenic callus were induced from whole embryo and epicotyl callus cultures on MS medium with 0.1 mg/L 2,4-D plus 1.0 mg/L BA. On the other hand, plenty of somatic embryos were obtained directly by culturing cotyledonary stage of immature embryos on MS media with 0.5 mg/L NAA plus 1.0 mg/L BA.
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참고문헌 (20)
참고문헌 신청
Agarwal BU / 1992 / In vitro clonal propagation of tea (Camellia sinensis) / Plant Cell Tissue Organ Cult 30:1~5
Ahn IS / 1995 / Somatic emgryogenesis and plant regeneration from immature embryo of Tea plant (Camellia sinensis) / Korean J. Breeding 27:90~91
Ana MV / 1990 / Somatic embryogenesis and plant regeneration from embryonic tissues of Camellia japonica L / Plant Cell, Tissue and Organ Culture 21:267~274
Ammirato PV / 1987 / Organizational events during somatic embryogenesis, In Plant tissue and cell culture, plant biology. vol. 3 / Alan R. Liss. :57~81
Bag N / 1997 / Mass propagation of tea using tissue culture methods / Physiol. Mol. Biol. Plants 3:99~103
Ahn IS / 1995 / Somatic emgryogenesis and plant regeneration from immature embryo of Tea plant (Camellia sinensis) / Korean J. Breeding 27:90~91
Ana MV / 1990 / Somatic embryogenesis and plant regeneration from embryonic tissues of Camellia japonica L / Plant Cell, Tissue and Organ Culture 21:267~274
Ammirato PV / 1987 / Organizational events during somatic embryogenesis, In Plant tissue and cell culture, plant biology. vol. 3 / Alan R. Liss. :57~81
Bag N / 1997 / Mass propagation of tea using tissue culture methods / Physiol. Mol. Biol. Plants 3:99~103
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