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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 332 - 338 (7page)
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초록· 키워드
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Microfluidic chips are versatile tools for automatic nucleic acid extraction and are very important for nucleic acid-based detection of pathogens in aqueous samples because polymerase chain reaction can amplify nucleic acids outside of the cell. In this study, we developed a simple and effective microfluidic chip platform for selectively extracting nucleic acid from bacteria and viruses by physical adsorption and bead beating. The sample was spiked with bacteria and virus, which were pseudo-selectively adsorbed onto the microbead in the first microchamber, whereas viruses in the output solution from the first chamber were adsorbed onto the surface of microbeads in second chamber. The bacteria and viruses were efficiently lysed in each chamber by microvalve-assisted bead beating following optimization of lysis conditions. The nucleic acids of viruses were well-separated in the first chamber and those of bacteria were isolated in the second chamber.
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참고문헌 (21)
참고문헌 신청
박재남 / 2010 / Multiplex detection of pathogens using an immunochromatographic assay strip / BioChip Journal 4(4):305~312
Kim, B. B. / 2007 / Advances in nucleic acid-based diagnostics of bacterial infections / Clinica Chimica Acta 384:1~11
Paul, T.M. / 2006 / Nucleic acid amplificationbased techniques for pathogen detection and identification / Infect. Genet. Evol 6:2~12
Erlich, H. A. / 1991 / Recent advances in the polymerase chain reaction / Science 252:1643~1651
Deiman, B. / 2002 / Characteristics and Applications of Nucleic Acid Sequence-Based Amplification (NASBA) / Mol. Biotechnol 20:163~180
Kim, B. B. / 2007 / Advances in nucleic acid-based diagnostics of bacterial infections / Clinica Chimica Acta 384:1~11
Paul, T.M. / 2006 / Nucleic acid amplificationbased techniques for pathogen detection and identification / Infect. Genet. Evol 6:2~12
Erlich, H. A. / 1991 / Recent advances in the polymerase chain reaction / Science 252:1643~1651
Deiman, B. / 2002 / Characteristics and Applications of Nucleic Acid Sequence-Based Amplification (NASBA) / Mol. Biotechnol 20:163~180
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