지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Background: Previous studies on Helicobacter pylori infection in mice have contributed to better understanding of the pathogenesis of chronic gastritis and gastric carcinoma. The aim of this study was to evaluate H. pylori colonization and subsequent inflammatory responses in the stomachs of C57BL/6 mice depending on inoculation number and the presence of high-salt diet.
Methods: Eighty-four female mice with 4 weeks age were used in this study. The infected mice were gavaged with H. pylori strain Sydney-1 (SS1), and the uninfected mice were dosed with vehicle. In each of these groups, half of the mice were fed ona basal diet (0.25% salt) and the other half were fed on a high-salt diet (7.5% salt). The infected mice were inoculated 4 or 5 times, and infection status and degree of inflammation were checked by culture and histopathology, respectively, after 4 weeks. Gastric mucosal myeloperoxidase and tumor necrosis factor-alpha were measured by ELISA.
Results: The overall infection rate was 95.2%; the infection rate after 5 inoculations (100%) was greater than that after 4 inoculations (91.3%). However, no differences in the degree of inflammation were found between 2 groups. The bacterial density was also significantly increased in mice that were on the high-salt diet and had been inoculated 5 times, respectively. Mean neutrophil infiltration in the infected group was 1.7±0.6 (1, minimal; 2, mild; 3, moderate; 4, marked). However, the high-salt diet was not increase the inflammatory grade in the infected group. Gastric mucosal myeloperoxidase and tumor necrosis factor-alpha levels did not increased by the high-salt diet and increased the number of inoculation.
Conclusions: In spite of well colonization of H. pylori in the stomachs of C57BL/6 mice, the degree of subsequent inflammation was irrelevant to high-salt diet and frequent (5 times) inoculations.
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참고문헌 (24)
참고문헌 신청
McColl KE / 2010 / Clinical practice. Helicobacter pylori infection / N Engl J Med 362:1597~1604
1994 / Infection with Helicobacter pylori / IARC Monogr Eval Carcinog Risks Hum 61:177~240
Tsukamoto T / 2013 / Helicobacter pylori infection and gastric carcinogenesis in rodent models / Semin Immunopathol 35:177~190
Lee A / 1990 / A small animal model of human Helicobacter pylori active chronic gastritis / Gastroenterology 99:1315~1323
Sakagami T / 1996 / Atrophic gastric changes in both Helicobacter felis and Helicobacter pylori infected mice are host dependent and separate from antral gastritis / Gut 39:639~648
1994 / Infection with Helicobacter pylori / IARC Monogr Eval Carcinog Risks Hum 61:177~240
Tsukamoto T / 2013 / Helicobacter pylori infection and gastric carcinogenesis in rodent models / Semin Immunopathol 35:177~190
Lee A / 1990 / A small animal model of human Helicobacter pylori active chronic gastritis / Gastroenterology 99:1315~1323
Sakagami T / 1996 / Atrophic gastric changes in both Helicobacter felis and Helicobacter pylori infected mice are host dependent and separate from antral gastritis / Gut 39:639~648
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