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- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.11
- 수록면
- 65 - 65 (1page)
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이 논문의 연구 히스토리 (2)
2021
Simultaneous analysis of multi-component lipids by ultra-high performance liquid chromatography-tandem mass spectrometry
이혜진
,
윤혜란
한국분석과학회 학술대회
2021.11
학술대회자료
Simultaneous analysis of multi-component lipids by ultra-high performance liquid chromatography-tandem mass spectrometry
이혜진
,
윤혜란
한국분석과학회 학술대회
2021.11
학술대회자료
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There is a growing interest in lipid metabolism-related chronic diseases such as asthma, chronic obstructive pulmonary disease to more severe diseases as forms of cancer.
We developed the ultrahigh performance liquid chromatography–electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) based analytical method of 40 lipids series (7 omega fatty acids, 3 sphingolipids, 23 glycerophospholipids and 5 neutral lipids) after “dried serum spot” sample preparation. We used “dried serum spot” sample preparation as an alternative method to conventional LLE or (SPE) methods. Developed method included special solvent system (MTBE:MeOH:DDW=3:2:1(v/v/v). After extraction, the lipid analytes were separated on a Acuity UPLC BEH C8 column using 5 mM ammonium acetate in 10% MeOH as mobile phase A and IPA:ACN:MeOH=2:3:5 as mob ... 전체 초록 보기
We developed the ultrahigh performance liquid chromatography–electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) based analytical method of 40 lipids series (7 omega fatty acids, 3 sphingolipids, 23 glycerophospholipids and 5 neutral lipids) after “dried serum spot” sample preparation. We used “dried serum spot” sample preparation as an alternative method to conventional LLE or (SPE) methods. Developed method included special solvent system (MTBE:MeOH:DDW=3:2:1(v/v/v). After extraction, the lipid analytes were separated on a Acuity UPLC BEH C8 column using 5 mM ammonium acetate in 10% MeOH as mobile phase A and IPA:ACN:MeOH=2:3:5 as mob ... 전체 초록 보기
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