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논문 기본 정보

자료유형
학술저널
저자정보
Xing Shan (가톨릭대학교) 최종현 (가톨릭대학교) 김기주 (가톨릭대학교) 이윤재 (가톨릭대학교) 류연희 (가톨릭대학교) 이수진 (가톨릭대학교) 문석호 (가톨릭대학교) 이종원 (가톨릭대학교)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제15권 제1호
발행연도
2018.1
수록면
49 - 61 (13page)

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This study was to investigate the effect of subcutaneous injection of the adipose stem cells (ASCs) with conditioned media (CM) in the treatment of acne vulgaris scar. We used Adult male New Zealand white rabbit ears as an animal model and induced acne formation by Kignman method. Adipose tissue was isolated and harvested from the scapula of rabbits, and ASCs were cultured and expanded until passage 1. There have four groups in our experiment, include phosphate buffered saline (PBS), ASCs with PBS (ASC ? PBS), CM, and ASCs with CM (ASC ? CM) group. This solution of 0.6 ml injected to subcutaneous in each group. ASC ? PBS and ASC ? CM groups were containing ASCs of 5.0 9 106 cells/ml. We analyzed the treatment of 4 groups to scar tissue after 2 and 4 weeks by hematoxylin and eosin stain, immunohistochemistry, and RNA expression level of tumor necrosis factor-a (TNF-a), interleukin-1a (IL-1a), and matrix metalloproteinase-2 (MMP-2). Also, the expression of keratin 16 (K16) was detected by western blot analysis. H&E stain showed that infiltration of inflammation cells was significantly reduced at 2 and 4 weeks, as well as re-epithelialization was improved in the ASC ? CM group. The ASC ? CM gourp was reduced both expression levels of TNF-a, IL- 1a, and MMP-2 and K16 protein level. In conclusion, the ASCs with CM has a significant curative effect on acne vulgaris scar, more to the point, the CM has a key role on treatment. It could be applied to a therapeutic approach to regenerate to treat acne vulgaris scar.

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