지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 277 - 287 (11page)
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초록· 키워드
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Intein-mediated protein engineering has become a valuable tool for biosensing applications. Expressed protein ligation and protein trans-splicing were used to generate proteins with necessary tags or cyclic peptides that can be used to build various biosensing platforms such as biochips or sensor proteins. Especially the use of split-inteins that carry out conditional protein splicing reaction enabled the fabrication of various genetically-encoded biosensors that can monitor various signaling events in vivo including protein- protein interactions and protein translocalization. Biological targets activated split-inteins to generate functioning reporter molecules, such as luciferase and autofluorescent proteins. We here review various sensing platforms that utilize intein-mediated protein engineering technology mainly focusing on cell-based biosensors.
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등록된 정보가 없습니다.
참고문헌 (53)
참고문헌 신청
Liu, Q. / 2014 / Cell-Based Biosensors and Their Application in Biomedicine / Chem. Rev 114:6423~6461
Ramachandran, N. / 2005 / Emerging tools for realtime label-free detection of interactions on functional protein microarrays / FEEB Journal 272:5412~5425
Liu, Y. / 2010 / Quantitative Analysis of Small Molecule-Nucleic Acid Interactions With a Biosensor Surface and Surface Plasmon Resonance Detection / Methods. Mol. Biol 613:1~23
Brian, R.B. / 2006 / An Electronic, Aptamer-Based Small-Molecule Sensor for the Rapid, Label-Free Detection of Cocaine in Adulterated Samples and Biological Fluids / J. Am. Chem. Soc 128:3138~3139
Zhou, W. / 2016 / In Vitro Selection in Serum: RNA-Cleaving DNAzymes for Measuring Ca2+ and Mg2+ / ACS Sens 1:600~606
Ramachandran, N. / 2005 / Emerging tools for realtime label-free detection of interactions on functional protein microarrays / FEEB Journal 272:5412~5425
Liu, Y. / 2010 / Quantitative Analysis of Small Molecule-Nucleic Acid Interactions With a Biosensor Surface and Surface Plasmon Resonance Detection / Methods. Mol. Biol 613:1~23
Brian, R.B. / 2006 / An Electronic, Aptamer-Based Small-Molecule Sensor for the Rapid, Label-Free Detection of Cocaine in Adulterated Samples and Biological Fluids / J. Am. Chem. Soc 128:3138~3139
Zhou, W. / 2016 / In Vitro Selection in Serum: RNA-Cleaving DNAzymes for Measuring Ca2+ and Mg2+ / ACS Sens 1:600~606
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